09260

Sigma-Aldrich

Atto 425-Streptavidin

BioReagent, suitable for fluorescence, ≥95% (HPCE)

Synonym(s):
Streptavidin-Atto 425
MDL number:
NACRES:
NA.32

Quality Level

product line

BioReagent

assay

≥95% (HPCE)

solubility

H2O: soluble

fluorescence

λex 437 nm; λem 477 nm in 0.1 M phosphate pH 7.0

suitability

corresponds for gel electrophoresis
suitable for fluorescence

storage temp.

−20°C

Application

Applied to the development of fluorescence binding assays to detect volatile organic compounds in both the liquid and gas phase.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Nanoscale organization of nicotinic aceylcholine receptors by stimulated emission depletion microscopy.
Kellner, R.R., et al.
Neuroscience, 144(1), 135-143 (2007)
Fluoreszierende Rhodamine und fluorogene Carbopyronine fur die STED-Mikroskopie lebender Zellen.
Butkevich, Alexey N.; et al.
Angewandte Chemie (International Edition in English), 128(10), 3350-3355 (2016)
Stimulated emission depletion-based raster image correlation spectroscopy reveals biomolecular dynamics in live cells.
Hedde P.N.; et al.
Nature Communications, 4, 2093-2093 (2013)
Synaptic membrane proteins form stable microdomains in early endosomes.
Geumann, U.; et al.
Microscopy Research and Technique, 73(6), 606-617 (2010)
STED Nanoscopy in Living Cells Using Fluorogen Activating Proteins.
Fitzpatrick, JA.; et al.
Bioconjugate Chemistry, 20(10), 1843-1847 (2009)
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