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10112001

COCA cell line from mouse

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Synonym(s):
COCA cells

biological source

mouse skin

Quality Level

growth mode

Adherent

karyotype

See Segrelles et al 2011

morphology

Epithelial

products

See Segrelles et al 2011

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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10112001

COCA cell line from mouse

vibrant-m

92101204

B16-F0 Cell Line from mouse

growth mode

Adherent

growth mode

Adherent

morphology

Epithelial

morphology

Epithelial

receptors

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

biological source

mouse skin

biological source

mouse skin

Cell Line Origin

Mouse epidermal keratinocyes, differentiation

Cell Line Description

COCA, a murine epidermal cell line was developed by serially passaging keratinocytes from the back skin of adult C57BL/DBA mice. Culture of the COCA cells is in fully defined media without requirement for feeder layer or other coating. COCA retains its ability to differentiate and stratify in response to increased calcium concentrations thus providing an excellent experimental system for in vitro (conventional and 3D epidermal cell cultures) and in vivo (skin regeneration) skin modelling.

Application

For complete product information, please see ECACC

Culture Medium

CnT-07 media This defined medium contains low calcium (0.07mM). To promote good cell attachment post trypsinisation, an 8-14 hours incubation in CnT-07 medium containing 0.2mM CaCl2 is necessary. Differentiation can be initiated by transferring a confluent monolayer from 0.07mM calcium to high calcium (1.2mM).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 0.5-1.5 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remov

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections, supplied by us, are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Carmen Segrelles et al.
BMC dermatology, 11, 9-9 (2011-04-23)
Skin diseases are a major health problem. Some of the most severe conditions involve genetic disorders, including cancer. Several of these human diseases have been modelled in genetically modified mice, thus becoming a highly valuable preclinical tool for the treatment

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