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102-05A

Sigma-Aldrich

Human Epidermal Keratinocytes: HEK, adult

NACRES:
NA.81

Quality Level

biological source

human neonatal foreskin or adult skin (normal)

packaging

pkg of 500,000 cells

growth mode

Adherent

karyotype

2n = 46

morphology

Epidermal

application(s)

cell culture | mammalian: suitable

relevant disease(s)

allergies; cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Epidermal keratinocytes comprise 90 % of all cells found in the epidermis, the outermost layer of skin. They produce keratin, the intermediate filament, largely responsible for skin′s barrier function. Through their interactions with epidermal melanocytes, keratinocytes obtain melanin and store it as a means of protection from UV radiation.

HEK provide an excellent model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology, as well as HPV infection.

When grown on inserts and provided with the liquid/air interface, HEK can differentiate into a stratified squamous epithelium and serve as a more physiological 3D tissue model for in vitro studies.

Cell Line Origin

Skin

Application

Skin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light response

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • Primary culture, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 16 doublings

Subculture Routine

Please refer to the HEK Culture Protocol.

Storage Class Code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Shunfeng Wang et al.
Molecules (Basel, Switzerland), 25(21) (2020-11-14)
In the present study, the fabrication of a biomimetic wound dressing that mimics the extracellular matrix, consisting of a hydrogel matrix composed of non-oxidized and periodate-oxidized marine alginate, was prepared to which gelatin was bound via Schiff base formation. Into...

Protocols

Human Epidermal Keratinocytes (HEK) Culture Protocol

Storage - Preparation for Culturing - Culturing HEK - Subculturing HEK

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