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10419

Sigma-Aldrich

8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate

for fluorescence, ≥95.0% (T)

Synonym(s):
1,8-ANS magnesium salt, ANSA magnesium salt, Magnesium 8-anilino-1-naphthalenesulfonate hydrate, N-Phenyl peri acid magnesium salt
Empirical Formula (Hill Notation):
C16H12Mg0.5NO3S · xH2O
CAS Number:
Molecular Weight:
310.49 (anhydrous basis)
Beilstein:
4834623
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

for fluorescence

Quality Level

Assay

≥95.0% (T)

form

powder

solubility

hot water: 50 mg/mL, green

λmax

465-475 nm

fluorescence

λex 388 nm; λem 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

O.[Mg++].[O-]S(=O)(=O)c1cccc2cccc(Nc3ccccc3)c12.[O-]S(=O)(=O)c4cccc5cccc(Nc6ccccc6)c45

InChI

1S/2C16H13NO3S.Mg.H2O/c2*18-21(19,20)15-11-5-7-12-6-4-10-14(16(12)15)17-13-8-2-1-3-9-13;;/h2*1-11,17H,(H,18,19,20);;1H2/q;;+2;/p-2

InChI key

FBANPIUXAXTAFX-UHFFFAOYSA-L

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This Item
10417A1028A5144
assay

≥95.0% (T)

assay

≥97.0% (HPLC)

assay

≥97% (HPLC)

assay

≥95% (perchloric acid titration)

form

powder

form

solid

form

powder

form

powder

solubility

hot water: 50 mg/mL, green

solubility

H2O: 0.1 g/5mL, clear to very slightly hazy (hot), NaOH: 1 N, H2O: soluble, acetone: soluble, methanol: soluble

solubility

DMF: 50 mg/mL

solubility

H2O: 50 mg/mL

λmax

465-475 nm

λmax

-

λmax

-

λmax

-

fluorescence

λex 388 nm; λem 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA

fluorescence

λex 388 nm; λem 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA

fluorescence

-

fluorescence

-

General description

ANSA (8-Anilino-1-naphthalenesulfonic acid) is a hydrophobic polarity sensitive fluorescent dye useful as a site probe to detect conformational changes in cell and micelle membranes and molecules such as proteins. ANSA is also used as a fluorescent probe for the estimation of the critical micelle concentration (CMC) of surfactants.

Features and Benefits

Fluorescent probe for the estimation of the CMC of surfactants.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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We have studied the metal-catalyzed oxidation (MCO) of brain-derived neurotrophic factor (BDNF) with regard to target sites and potential conformational changes of the protein. The exposure of BDNF to three different levels of ascorbate/Cu(II)/O2 [20 microM Cu(II), 2 mM ascorbate

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