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106-05A

Sigma-Aldrich

Human Dermal Fibroblasts: HDF, adult

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Synonym(s):
HDF cells
NACRES:
NA.81

biological source

human neonatal foreskin or adult skin (normal)

Quality Level

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 46

morphology

Fibroblast

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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This Item
106-05N106-05F602-05A
biological source

human neonatal foreskin or adult skin (normal)

biological source

human neonatal foreskin or adult skin (normal)

biological source

human neonatal foreskin or adult skin (normal)

biological source

human scalp (hair follicle papilla)

growth mode

Adherent

growth mode

Adherent

growth mode

Adherent

growth mode

Adherent

morphology

Fibroblast

morphology

Fibroblast

morphology

Fibroblast

morphology

mesenchymal

karyotype

2n = 46

karyotype

2n = 46

karyotype

2n = 46

karyotype

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)

Cell Line Origin

Skin

Application

injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response

Components

Fibroblast Basal Medium containing 10% FBS & 10% DMSO.

Preparation Note

  • Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO.
  • Can be cultured at least 16 doublings.

Culture Medium

Fibroblast Growth Medium (116-500) all-in-one ready to use media

Subculture Routine

Please refer to the HDF Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

required but not provided

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Manthan Patel et al.
BMC genetics, 21(1), 84-84 (2020-07-31)
The human CGGBP1 binds to GC-rich regions and interspersed repeats, maintains homeostasis of stochastic cytosine methylation and determines DNA-binding of CTCF. Interdependence between regulation of cytosine methylation and CTCF occupancy by CGGBP1 remains unknown. By analyzing methylated DNA-sequencing data obtained
Michele Galluccio et al.
Molecular biology reports, 47(9), 7283-7289 (2020-08-11)
It is well established that Escherichia coli represents a powerful tool for the over-expression of human proteins for structure/function studies. In many cases, such as for membrane transporters, the bacterial toxicity or the aggregation of the target protein hamper the
Lucie Peterková et al.
Tissue & cell, 58, 121-129 (2019-05-28)
Surface modification is an important step in making a synthetic polymer cytocompatible. We have previously reported improved cytocompatibility of immortalized human keratinocytes (HaCaT) with the otherwise bioinert fluorinated ethylene propylene (FEP) upon treatment with argon plasma discharge. In this article

Protocols

This protocol contains: Storage - Preparation for Culturing - Culturing HDF - Subculturing HDF

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