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10771

Sigma-Aldrich

Histopaque®-1077

sterile-filtered, density: 1.077 g/mL

NACRES:
NA.75

sterility

sterile-filtered

Quality Level

shelf life

Expiry date on the label.

impurities

endotoxin, tested

density

1.077 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

General description

Histopaque-1077 is a sterile, endotoxin tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood.

Application

Histopaque-1077 is suitable for human lymphocyte antigen (HLA) typing and as the initial isolation step prior to the enumeration of T, B, and ‘null’ lymphocytes. It may also be employed in the preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.

Histopaque-1077 has been used for-

  • the isolation of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)
  • purification of mononuclear cells through centrifugation
  • isolation of human lymphocytes
  • isolation of breast cancer circulating tumor cells (CTCs) from the whole blood

Packaging

100, 100, 500 mL
10 L
1 gal

Legal Information

Histopaque is a registered trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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