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11012001

FLO-1 cell line human

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Synonym(s):
FLO1 Cells

biological source

human esophagus

Quality Level

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

P53 mutation c.830G>T

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

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FLO-1 cell line human

11012001

FLO-1 cell line human

KYAE-1 cell line

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KYAE-1 cell line

ESO51 cell line human

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ESO51 cell line human

SK-GT-4 cell line human

11012007

SK-GT-4 cell line human

receptors

Not specified

receptors

Not specified

receptors

Not specified

receptors

Not specified

karyotype

Not specified

karyotype

Not specified

karyotype

Not specified

karyotype

Modal number 59 chromosomal aberration at 11p13-15.

growth mode

Adherent

growth mode

Adherent

growth mode

Suspension

growth mode

Adherent

biological source

human esophagus

biological source

human esophagus

biological source

human esophagus

biological source

human esophagus

morphology

Epithelial

morphology

Epithelial

morphology

Large spherical clumps of cells growing in suspension

morphology

Epithelial growing in islands

Cell Line Origin

Distal oesophageal adenocarcinoma

Application

For complete product information, please see ECACC

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 12,13
D5S818: 12,14
D7S820: 8
THO1: 6
TPOX: 9,11
vWA: 16

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 105 cells/cm2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Sarah Derks et al.
Cancer immunology research, 3(10), 1123-1129 (2015-06-18)
Esophageal adenocarcinoma is an increasingly common disease with a dismal 5-year survival rate of 10% to 15%. In the first systematic evaluation of the PD-1 pathway in esophageal adenocarcinoma, we identify expression of PD-L2 in cancer cells in 51.7% of
S J Hughes et al.
Cancer research, 57(24), 5571-5578 (1998-01-04)
This study describes Fas (CD95) expression in Barrett's esophagus, adenocarcinomas of the esophagus, and three esophageal adenocarcinoma cell lines. Immunohistochemical analysis of Barrett's esophagus demonstrated cell surface expression of Fas protein. In contrast, 30.5% of esophageal adenocarcinomas examined by immunohistochemical
Mathieu Francois Derouet et al.
PloS one, 9(12), e115589-e115589 (2015-01-01)
Carcinoma of the esophagus has a high case fatality ratio and is now the 6th most common cause of cancer deaths in the world. We previously conducted a study to profile the expression of miRNAs in esophageal adenocarcinoma (EAC) pre
Jurjen J Boonstra et al.
Journal of the National Cancer Institute, 102(4), 271-274 (2010-01-16)
For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the

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