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11012002

Sigma-Aldrich

KYAE-1 cell line

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biological source

human esophagus

Quality Level

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

P53 mutation c.455C>T

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Distal oesophageal adenocarcinoma,

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 11
D5S818: 10,13
D7S820: 11,12
THO1: 6
TPOX: 8
vWA: 14

Culture Medium

RPMI-1640:Hams F12 1:1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. There is a requirement to change the culture medium every 2-3 days.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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