12133C

Sigma-Aldrich

Bovine Calf Serum

USA origin, sterile-filtered, suitable for cell culture

Synonym(s):
Calf Serum, calf sera, calf serum, bovine calf sera
MDL number:

Quality Level

biological source

bovine (calf)

sterility

sterile-filtered

composition

hemoglobin, ≤35 mg/dL

origin

USA origin

application(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro. Bovine Calf Serum (BCS) is collected from calves that are less than 12 months old. The serum contains approximately double the protein of fetal bovine serum (FBS). BCS will promote growth in a variety of mammalian cell lines and is often used as a cost-effective substitute for FBS.

Application

Bovine Calf Serum has been used as a basal media supplement for growing :
  • Immunocompetent cells of ileal Peyer′s patches
  • Mouse skeletal myoblasts
  • Ovarian cell lines
  • Bovine retinal pericytes
Bovine Calf Serum has been used as a basal media supplement for growing:
  • immunocompetent cells of ileal Peyer′s patches
  • mouse skeletal myoblasts
  • ovarian cell lines
  • bovine retinal pericytes

Use in the propogation of viruses, Helicobacter pylori and Plasmodium falciparum.

Biochem/physiol Actions

Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro. Bovine Calf Serum (BCS) is collected from calves that are less than 12 months old. The serum contains approximately double the protein of fetal bovine serum (FBS). BCS will promote growth in a variety of mammalian cell lines and is often used as a cost-effective substitute for FBS.

Testing Method

Endotoxin tested

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Coculture Assays
for Endothelial Cells-Mural Cells Interactions
Sanchez Palencia DM, et al.
Tumor, 35-47 (2016)
Coculture Assays for Endothelial Cells-Mural Cells Interactions
Tumor Angiogenesis Assays, 35-47 (2016)
Sung Soo Kim et al.
Journal of Korean medical science, 17(3), 302-306 (2002-06-18)
In vitro subcultures of bacteria can lead to genetic and phenotypic changes. This study aimed at investigating the effect of repeated subcultures on the adhesion, motility, cytotoxicity, and gastric inflammation caused by Helicobacter pylori. H.pylori SS1 strain was subcultured 64...
The effect
of in vivo exposure to zearalenone on cytokine secretion by Th1 and Th2 lymphocytes in porcine Peyer?s patches after in vitro stimulation with LPS
Obremski K
Polish Journal of Veterinary Sciences, 17(4), 625-632 (2014)
D V Young et al.
In vitro, 16(11), 949-957 (1980-11-01)
Simian virus 40-transformed 3T3 cells are dependent on serum for survival and growth. This growth activity can be separated on a pH 2 Sephadex G100 column into two fractions: a high molecular weight activity and a low molecular weight substance...
Articles
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
Read More

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