12138C

Bovine Calf Serum

Name: Bovine Calf Serum
Brand: SIGMA

Iron-Fortified, USA origin, from formula-fed bovine calves, sterile-filtered, suitable for cell culture

Synonym(s):

Calf Serum, BCS, bovine calf sera, calf sera, calf serum

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About This Item

MDL number:

MFCD00243108

UNSPSC Code:

12352207

NACRES:

NA.71

Biological source:

bovine (calf) (formula-fed)

Origin:

USA origin

Sterility:

sterile-filtered

Shipped in:

dry ice

Key Documents

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biological source

bovine (calf) (formula-fed)

Quality Level

500

sterility

sterile-filtered

composition

hemoglobin, ≤35 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Show Differences

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This Item	C8056	13067C	N4762
Sigma-Aldrich 12138C Bovine Calf Serum	Sigma-Aldrich C8056 Calf Serum	Sigma-Aldrich 13067C Bovine Calf Serum	Sigma-Aldrich N4762 Newborn Calf Serum
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
origin USA origin	origin USA origin	origin Australia origin	origin USA origin
sterility sterile-filtered	sterility sterile-filtered	sterility γ-irradiated by SER-TAIN^™ process, sterile-filtered	sterility sterile-filtered
biological source bovine (calf) (formula-fed)	biological source bovine (calf) (formula-fed)	biological source bovine (calf)	biological source bovine (calf)
composition hemoglobin, ≤35 mg/dL	composition hemoglobin, ≤35 mg/dL	composition Hemoglobin, ≤35 mg/dL	composition hemoglobin, ≤20 mg/mL
impurities ≤10 EU/mL endotoxin	impurities ≤10 EU/mL Endotoxin	impurities ≤50 EU/mL Endotoxin	impurities ≤100 EU/mL endotoxin

Application

Endotoxin tested

Iron-supplemented bovine serum as an alternative to fetal bovine serum in the CHO/HGPRT mutation assay, Oberly, et. al. Development of a more rapid, reduced serum culture system for Caco-2 monolayers and application to the biopharmaceutics classification system. Lentz et. al.

Preparation Note

Each lot is supplemented to a constant iron concentration.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

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Iron-supplemented bovine serum as an alternative to fetal bovine serum in the CHO/HGPRT mutation assay.

T J Oberly et al.

Mutation research, 244(2), 105-109 (1990-06-01)

Iron-supplemented bovine calf serum (ICS) was found to be a viable alternative to fetal bovine serum (FBS) in the growth promotion and cloning efficiency of Chinese hamster ovary (CHO) cells that are used in the HGPRT mutation assay. Suspension cultures

Comparison of fortified calf serum, serum substitutes and fetal calf serum with or without extenders for propagation of cell cultures for virus plaque assays.

D R Dahling et al.

Journal of virological methods, 27(3), 287-294 (1990-03-01)

Two studies comparing sewage-isolated and laboratory stock viruses were conducted to determine if alternative forms of serum or serum extenders could be used in place of fetal bovine serum without a significant loss of viral titer. In the first study

Development of a more rapid, reduced serum culture system for Caco-2 monolayers and application to the biopharmaceutics classification system.

K A Lentz et al.

International journal of pharmaceutics, 200(1), 41-51 (2000-06-14)

The objectives were: (1) to develop a more rapid, reduced serum culture system for Caco-2 monolayers, relative to the traditional 21-day, 10% fetal bovine serum (FBS) system; and (2) to determine the biopharmaceutical drug classification of an oral therapeutic agent

Sphingolipids are required for efficient triacylglycerol loss in conjugated linoleic Acid treated adipocytes.

Wei Wang et al.

PloS one, 10(4), e0119005-e0119005 (2015-04-24)

Conjugated linoleic acid (CLA) reduces adiposity in human and mouse adipocytes. This outcome is achieved through a variety of biological responses including increased energy expenditure and fatty acid oxidation, increased inflammation, repression of fatty acid biosynthesis, attenuated glucose transport, and

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