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Sigma-Aldrich

Serum Plus II Medium Supplement

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sterility

sterile-filtered

shipped in

dry ice

storage temp.

−20°C

General description

Serum Plus II Medium Supplement contains a low level of Fetal Bovine Serum (FBS) in a proprietary formulation to promote cell attachment and cell growth.

Application

Serum Plus II Medium Supplement is a proprietary formulation developed for researchers seeking Fetal Bovine Serum alternatives. Serum Plus II is a medium supplement that can be used as a substitute for fetal bovine serum in most cell culture applications.

Features and Benefits

Benefits include reduced levels of inhibitors, improved lot-to-lot performance due to better control of component materials and reduced cost compared to fetal bovine serum. This lower-cost option has been tested on a range of commonly-used cell lines and it has performed equally (and sometimes better) than Fetal Bovine Serum.

Preparation Note

Thawing
1. Remove the supplement bottles from the freezer and allow them to acclimate to room temperature for approximately 10 minutes.
2. Place each container in a 30 - 37 °C water bath or incubator. Excessive temperatures will degrade heat liable nutrients. If using a water bath, prevent the bottle caps from being completely submerged.
3. Gently swirl or shake the bottles every 10-15 minutes until the serum is completely thawed.
4. After thawing, use the supplement promptly. Liquid supplement may be stored refrigerated (2 - 8 °C) up to four weeks. To avoid freeze/thaw cycles or long periods of refrigeration, it is recommended that any unused supplement be immediately dispensed into small, useful aliquots and refrozen for future use.

Note:
Periodic agitation is crucial for optimum performance. If a bottle is not periodically shaken or swirrled as it thaws, gradients containing high concentrations of salts, proteins and lipids will form throughout the liquid portion and lead to the formation of crystalline or flocculent precipitates. These cryoprecipitates are not toxic to cell cultures, but they affect the appearance and consistency of each bottle.

Small amounts of cryoprecipitates are not uncommon, and will not affect product performance. Gently warming and mixing the supplement will generally allow the material to go back into solution.

Legal Information

SERUM PLUS is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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