EX-CELL® 293 Serum-Free Medium for HEK 293 Cells

Serum-free, Animal-protein free, without L-glutamine, liquid, suitable for cell culture

MDL number:
Pricing and availability is not currently available.

Quality Level


for research or for further manufacturing use





storage condition

protect from light


cell culture | mammalian: suitable


glucose: 6.0 g/L
NaHCO3: 1.8 g/L
L-glutamine: no

shipped in


storage temp.


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EX-CELL 293 is an animal-protein free, serum-free medium developed for the long-term growth of Human Embryonic Kidney 293 (HEK 293) and related cells. The cells, in a suspension culture, can be subcultured directly into EX-CELL 293 from serum-supplemented media with little or no adaptation. Suspension cultures can be maintained, without refeeding, for about 10 days and can be carried for more that 20 passages with no loss of viability.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Jan Bláha et al.
Protein expression and purification, 109, 7-13 (2015-01-28)
Lectin-like transcript 1 (LLT1, gene clec2d) was identified to be a ligand for the single human NKR-P1 receptor present on NK and NK-T lymphocytes. Naturally, LLT1 is expressed on the surface of NK cells, stimulating IFN-γ production, and is up-regulated...
Pratik Jaluria et al.
Biotechnology and bioengineering, 99(6), 1443-1452 (2007-11-21)
Animal-derived serum is an essential media supplement for mammalian cells in cell culture. For a number of reasons including cost, regulatory concerns, lot inconsistency, potential contamination with adventitious agents, and down-stream processing it is desirable to eliminate the use of...
G Schoofs et al.
Cytotechnology, 28(1-3), 81-89 (2008-11-13)
We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum...
Jan Bláha et al.
Protein expression and purification, 140, 36-43 (2017-08-02)
Human natural killer receptor protein 1 (NKR-P1, CD161, gene klrb1) is a C-type lectin-like receptor of natural killer (NK) cells responsible for recognition of its cognate protein ligand lectin-like transcript 1 (LLT1). NKR-P1 is the single human orthologue of the...
J B Kaufman et al.
Cytotechnology, 33(1-3), 3-11 (2008-11-13)
The extracellular domain of human parathyroid Ca(2+) receptor was needed in order to study itsstructure and clinical application. The Ca(2+)receptor is a unique member of the G protein-coupledreceptor super-family, expressed in parathyroid andkidney cells where it has been shown to...
Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines at sigmaaldrich.com
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