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14610C

SAFC

EX-CELL® 610-HSF Serum-Free Medium for Hybridoma Cells

Low-protein, with L-glutamine, liquid, sterile-filtered, suitable for cell culture

MDL number:
NACRES:
NA.75

description

Drug Master File available
for research or for further manufacturing use

Quality Level

sterility

sterile-filtered

form

liquid

storage condition

protect from light

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

components

NaHCO3: 1.60 g/L
HEPES: 7.5 mM
glucose: 3.45 g/L
L-glutamine: 0.333 g/L

shipped in

ambient

storage temp.

2-8°C

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Application

EX-CELL 610-HSF is a low-protein (11 mg/L) serum-free medium. Originally developed to support the growth of hybrid cells in culture, it has been shown to support a wide range of cells including lymphoid and epithelial cells and B cell hybridomas of murine, rat and human origin. EX-CELL 610-HSF has been used in stationary culture systems and in large-scale bioreactors. In both types of culture, the production of cellular products, particularly monoclonal antibodies, has been shown to equal or exceed levels seen when the same cells are cultured in the presence of Fetal Bovine Serum (FBS).

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Gunasekaran Dhandapani et al.
mAbs, 11(3), 583-592 (2019-01-09)
We introduce a new concept and potentially general platform for antibody (Ab) purification that does not rely on chromatography or specific ligands (e.g., Protein A); rather, it makes use of detergent aggregates capable of efficiently capturing Ab while rejecting hydrophilic

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