14918

Sigma-Aldrich

Atto 655 amine

suitable for fluorescence

NACRES:
NA.32

Quality Level

fluorescence

λex 663; λem 684 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 655 belongs to a new generation of fluorescent labels. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, good fluorescence quantum yield, excellent thermal and photo-stability, outstanding ozone resistance, very good water solubility, and very little triplet formation. Atto 655 is a zwitterionic dye with a net electrical charge of zero. The fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc.
The amine derivative may be used for reactions with activated carboxy-groups like NHS-esters, TFP-esters etc.

find more information
here

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Suitability

for coupling to activated carboxy groups

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Zhixing Chen et al.
Journal of the American Chemical Society, 134(33), 13692-13699 (2012-08-10)
Chemical tags are now viable alternatives to fluorescent proteins for labeling proteins in living cells with organic fluorophores that have improved brightness and other specialized properties. Recently, we successfully rendered our TMP-tag covalent with a proximity-induced reaction between the protein...
Katharina Stöhr et al.
Analytical chemistry, 77(22), 7195-7203 (2005-11-16)
Due to growing problems with new emerging pathogens, cost-effective and manageable methods for their accurate identification in routine diagnostics are urgently required. Of particular importance is the genus Mycobacterium with its more than 100 species. Identification of these species is...
Fabian Heinemann et al.
Langmuir : the ACS journal of surfaces and colloids, 28(37), 13395-13404 (2012-08-16)
Fluorescence correlation spectroscopy (FCS) measurements are widely used for determination of diffusion coefficients of lipids and proteins in biological membranes. In recent years, several variants of FCS have been introduced. However, a comprehensive comparison of these methods on identical systems...
Sridharan Rajagopalan et al.
Nucleic acids research, 39(6), 2294-2303 (2010-11-26)
The state of oligomerization of the tumor suppressor p53 is an important factor in its various biological functions. It has a well-defined tetramerization domain, and the protein exists as monomers, dimers and tetramers in equilibrium. The dissociation constants between oligomeric...
Julie M G Rogers et al.
Langmuir : the ACS journal of surfaces and colloids, 27(7), 3815-3821 (2011-03-16)
The structure and function of the influenza A M2 proton channel have been the subject of intensive investigations in recent years because of their critical role in the life cycle of the influenza virus. Using a truncated version of the...

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