150-05A

Sigma-Aldrich

Human Skeletal Muscle Cells: HSkMC, adult

NACRES:
NA.81

biological source

human skeletal muscle (normal limb)

Quality Level

packaging

pkg of 500,000 cells

growth mode

Adherent

karyotype

2n = 46

morphology

Skeletal muscle

application(s)

cell culture | mammalian: suitable

relevant disease(s)

muscular dystrophy

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of limbs from adult or fetal donors. Human Skeletal Muscle Cells can undergo differentiation to exhibit actin and myosin myofilaments. HSkMC from Cell Applications, Inc. provide a useful model system to study many aspects of muscular function and disease.Skeletal Muscle Cells play an instrumental role in the glucose metabolism and diabetes, therefore select HSkMC lots undergo an additional set of characterization at Cell Applications, Inc. to demonstrate AMPK signaling and responsiveness to insulin stimulation.

Cell Line Origin

Muscle (Limb)

Application

lipid and glucose metabolism, exercise, insulin action, cell signaling

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 15 doublings

Subculture Routine

Please refer to the HSkMC Culture Protocol.

RIDADR

NONH for all modes of transport

Certificate of Analysis
Certificate of Origin
Protocols
Technical information for working with human skeletal muscle cells including thawing, subculturing and cryopreservation
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