150-05F

Human Skeletal Muscle Cells: HSkMC, fetal

• NACRES: NA.81

Properties

• biological source: human skeletal muscle (normal limb)
• Quality Level: 100
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Skeletal muscle
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): muscular dystrophy
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of limbs from adult or fetal donors. Human Skeletal Muscle Cells can undergo differentiation to exhibit actin and myosin myofilaments. HSkMC provide a useful model system to study many aspects of muscular function and disease. Skeletal Muscle Cells play an instrumental role in the glucose metabolism and diabetes, therefore select HSkMC lots undergo an additional set of characterization to demonstrate AMPK signaling and responsiveness to insulin stimulation.

Cell Line Origin

Muscle (Limb)

Packaging

1 each

Application

lipid and glucose metabolism, exercise, insulin action, cell signaling

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

• 3rd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 15 doublings

Subculture Routine

Please refer to the HSkMC Culture Protocol.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable