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Brij® L23 solution

~10% in H2O

C12E23, Brij® 35 solution, Polyoxyethylene (23) lauryl ether
CAS Number:
MDL number:
PubChem Substance ID:




~10% in H2O

aggregation number



≤150 μM sulfhydryl oxidizing substances (carbonyls, peroxides)

refractive index

n20/D 1.347


91 μM

transition temp

cloud point ≥100 °C


ethanol: soluble
mineral oil: miscible
toluene: soluble
water: soluble


1.01 g/mL at 20 °C

cation traces

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤50 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤500 mg/kg
Mg: ≤50 mg/kg
Mn: ≤5 mg/kg
Na: ≤500 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg



SMILES string




InChI key


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Brij®L23 solution is a detergent used for measuring the concentration of carbon black in lung tissues. It has also been used for selective and rapid solubilization of microbial membrane aldehyde dehydrogenase.

Biochem/physiol Actions

Brij®L23 also referred to as Brij 35 or Polyoxyethylene (23) lauryl ether is a non-ionic detergent that lowers the non-specific protein adsorption and reduces electroosmotic pumping drastically. This allows the pH to be varied without adversely affecting the flow rate. It also used as a nonionic surfactant that decreases the absorbance of the blank, inhibits turbidity as well as provides linearity.


package with 6 x 10 ml ampoules

Legal Information

Brij is a registered trademark of Croda International PLC

Certificate of Analysis

Certificate of Origin

B T Dumas et al.
Clinica chimica acta; international journal of clinical chemistry, 258(1), 21-30 (1997-02-03)
A rapid and reliable method for measuring serum albumin employing bromcresol green is described. The addition of albumin to a solution of bromcresol green in a 0.075 M succinate buffer pH 4.20 results in an increase in absorbance at 628...
J K Towns et al.
Analytical chemistry, 63(11), 1126-1132 (1991-06-01)
Capillary zone electrophoretic separations of proteins have been achieved by using nonionic surfactant coated capillaries. Capillaries were prepared by derivatization of the silica surface with octadecylsilane followed by the deposition of a layer of nonionic surfactant from an aqueous solution...
Cheol-Hong Lim et al.
Environmental health and toxicology, 28, e2013014-e2013014 (2013-12-05)
This study was conducted to determine whether nano-sized carbon black exposure results in greater damage in high fat diet-induced overweight rats than normal weight ones and to identify the possible causes of any differences. Two groups of Sprague-Dawley rats allocated...
R Hommel et al.
Journal of basic microbiology, 30(4), 297-300 (1990-01-01)
An improved solubilization procedure for the membrane-bound quinoprotein aldehyde dehydrogenase from Acetobacter rancens CCM 1774 was established. After the first solubilization of membrane enzymes by Brij 35 which provided important extraction of membrane proteins other than aldehyde dehydrogenase, the application...
Darío Morais et al.
Rhinology, 50(1), 80-86 (2012-04-04)
Hereditary Haemorrhagic Telangiectasia or Rendu-Osler-Weber syndrome is a rare autosomal dominant vascular disease characterized by mucocutaneous and gastrointestinal telangiectases and localized arteriovenous malformations in lung, brain and liver. Epistaxis, due to rupture of telangiectases of the nasal mucosa, is the...

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