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Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside

≥98.0% (HPLC)

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Synonym(s):
X-α-Gal
Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
Molecular Weight:
408.63
MDL number:
PubChem Substance ID:
NACRES:
NA.32

assay

≥98.0% (HPLC)

form

powder or crystals

optical activity

[α]/D 135.0±5.0°, c = 1 in DMF/H2O 1:1

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14+/m1/s1

InChI key

OPIFSICVWOWJMJ-YGEXGZRRSA-N

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This Item
B4252B4387B9146
Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

form

powder or crystals

form

powder

form

powder

form

powder

optical activity

[α]/D 135.0±5.0°, c = 1 in DMF/H2O 1:1

optical activity

-

optical activity

-

optical activity

-

Application

5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside is suitable as a substrate for α-galactosidase. It has been used:
  • in the yeast two-hybrid screen
  • for differentiating α-galactosidase-positive strains of yeast
  • to determine melibiase activity in yeast and to identify Saccharomyces cerevisiae A241 × Saccharomyces uvarum C995 hybrids

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Tubb, R.S. and Liljestrom, P.L.
Journal of the Institute of Brewing, 92, 588-588 (1986)
A novel reporter gene MEL1 for the yeast two-hybrid system.
S Aho et al.
Analytical biochemistry, 253(2), 270-272 (1997-12-31)
A Unique Saccharomyces cerevisiae ? Saccharomyces uvarum Hybrid Isolated From Norwegian Farmhouse Beer: Characterization and Reconstruction
Krogerus K, et al.
Frontiers in Microbiology, 9 (2018)
Jiaojie Li et al.
Cells, 8(12) (2019-11-28)
Amyotrophic lateral sclerosis (ALS) caused by mutation of superoxide dismutase 1 (SOD1), affects various cellular processes and results in the death of motor neurons with fatal defects. Currently, several neurological disorders associated with DNA damage are known to directly induce
Florence Teulé et al.
Nature protocols, 4(3), 341-355 (2009-02-21)
The extreme strength and elasticity of spider silks originate from the modular nature of their repetitive proteins. To exploit such materials and mimic spider silks, comprehensive strategies to produce and spin recombinant fibrous proteins are necessary. This protocol describes silk

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