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Sigma-Aldrich

Atto 680-Streptavidin

BioReagent, suitable for fluorescence

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Synonym(s):
Streptavidin-Atto 680
MDL number:
NACRES:
NA.32

product line

BioReagent

Quality Level

form

powder

manufacturer/tradename

ATTO-TEC GmbH

solubility

H2O: soluble (readily soluble)

fluorescence

λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0

suitability

corresponds for gel electrophoresis
suitable for fluorescence

storage temp.

−20°C

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vibrant-m

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Atto 680-Streptavidin

vibrant-m

40709

Atto 590-Streptavidin

vibrant-m

94149

Atto 647N-Streptavidin

vibrant-m

49937

Atto 488-Streptavidin

fluorescence

λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 590 nm; λem 619 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

BioReagent

form

powder

form

powder

form

powder

form

solid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

Atto 680 is a fluorescent label with high molecular absorption (125.0) and quantum yield (0.30), as well as a sufficient Stoke′s shift (excitation maximum 680 nm, emission maximum 700 nm). It has limited cis-trans-isomerization, which enhances its brightness and reproducibility. Conjugation of Atto 680 to steptavidin does not significantly alter the label′s spectral data regarding excitation and emission maxima. Additionally, the molar absorbance and quantum yield remain high. Such conjugation is useful as a secondary detection reagent in histochemical applications, flow cytometry, microarrays, blot analysis, and immunoassays. Atto 680-streptavin does not show strong fluorescence until bound to biotin.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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