16805

Atto 425 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

• Synonym(s): Atto 425-N-hydroxysuccinimide ester
• Empirical Formula (Hill Notation): C₂₆H₃₀N₂O₈
• CAS Number: 892156-28-4
• Molecular Weight: 498.53
• MDL number: MFCD04974819
• PubChem Substance ID: 57647590
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 100
• assay: ≥90% (HPLC); ≥90% (degree of coupling)
• manufacturer/tradename: ATTO-TEC GmbH
• solubility: DMF: soluble; DMSO: soluble; H₂O: soluble
• λ: in ethanol (with 0.1% trifluoroacetic acid)
• UV absorption: λ: 422-428 nm A_max
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(=O)ON4C(=O)CCC4=O)c3cc2OC1=O
• InChI: 1S/C26H30N2O8/c1-5-34-24(32)18-12-16-11-17-15(2)14-26(3,4)27(19(17)13-20(16)35-25(18)33)10-6-7-23(31)36-28-21(29)8-9-22(28)30/h11-13,15H,5-10,14H2,1-4H3
• InChI key: PUEQEMDTFPYCDY-UHFFFAOYSA-N

Description

General description

Atto 425 is a member of the commercially available coumarin derivatives.[1]

Application

Atto 425 NHS ester is used in the development of bifunctional fluorescent, inorganic micro-partices (Zeolite L crystals) designed for targeting or imaging mutliple components . Reacts with amino groups or amine modified oliognucleotides to form a stable amine bond. Utilized as an essential component of molecular beacons, which were designed for use in real-time PCR assays to allow for rapid and accurate identification of gram-negative bacteria . When conjugated with benzylguanine, the fluorescence of Atto 425-NHS is quenched but becomes fluorescent upon labeling of the SNAP-tag fusion protein, making this conjugate an ideal candidate for experiments in living cells . Used as one of the donor probes in three-color FRET experiments designed to investigate the kinetics of RNA-protein complex formation .

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Other Notes

Study of stability during one- and two-photon excitation in fluorescence correlation spectroscopy, FCS[2]; employed in triple-color coincidence analysis[3].

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves