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16986

Sigma-Aldrich

Atto 700 NHS-ester

BioReagent, suitable for fluorescence, ≥90% (coupling to amine, HPLC)

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MDL number:
MFCD06798564
NACRES:
NA.32

product line

BioReagent

Quality Level

100

Assay

≥90% (coupling to amine, HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
700 nm

fluorescence

λex 681 nm; λem 713 nm in 0.1 M phosphate pH 7.0

λ

(ethanol with 0.1% trifluoroacetic acid)

UV absorption

λ: 687- 693 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

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assay

≥90% (coupling to amine, HPLC)

assay

≥85% (HPLC)

assay

≥90% (coupling to thiols)

assay

≥90% (HPLC), ≥90% (degree of coupling)

form

powder

form

powder

form

solid

form

powder

fluorescence

λex 681 nm; λem 713 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 647 nm; λem 669 nm in 0.1 M phosphate pH 7.0

fluorescence

-

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Jan Vogelsang et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 8(4), 486-496 (2009-04-02)
The role and interplay of triplet states and radical ion states in single-molecule fluorescence spectroscopy has recently been elaborated providing us with new insights into the photophysics and photobleaching pathways of fluorescent dyes. Adjustment of fluorophore redox properties in combination
Patricia Haus et al.
Journal of biomolecular screening, 16(10), 1206-1216 (2011-10-27)
Histone deacetylases (HDACs) are important epigenetic factors regulating a variety of vital cellular functions such as cell cycle progression, differentiation, cell migration, and apoptosis. Consequently, HDACs have emerged as promising targets for cancer therapy. The drugability of HDACs has been
Daniel Riester et al.
Bioorganic & medicinal chemistry letters, 19(13), 3651-3656 (2009-05-22)
Histone deacetylases reside among the most important and novel target classes in oncology. Selective lead structures are intensively developed to improve efficacy and reduce adverse effects. The common assays used so far to identify new lead structures suffer from many
Sebastian van de Linde et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 8(4), 465-469 (2009-04-02)
We introduce a general approach for multicolor subdiffraction-resolution fluorescence imaging based on photoswitching of standard organic fluorophores. Photoswitching of ordinary fluorophores such as ATTO520, ATTO565, ATTO655, ATTO680, or ATTO700, i.e. the reversible transition from a fluorescent to a nonfluorescent state

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