λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+
suitable for fluorescence
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Sigma tests the solubility of Product No. 17783 at a concentration of 10 mg/mL in dimethyl sulfoxide (DMSO).
Product No. 17783 can be a film, oil, paste or solid.
The emission maximum for Calcein-AM is 516 nm, with excitation at 496 nm in 0.1M Tris pH 8.0 (following hydrolysis by esterase enzyme, bound to Ca(2+)).
Calcein-AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability. In live cells the non-fluorescent calcein-AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells.
Although Bel-Art does not specifically test their Scienceware® brand polypropylene products, they have no evidence of any problems with leachable material in the polypropylene.
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