The CryptoFluor dyes are phycobiliproteins isolated from cryptomonad algae that provide additional spectral characteristics to complement the more common phycobiliproteins (e.g., R-PE and APC) as well as providing much smaller molecular weights (around 40,000 Daltons). These characteristics are meant to provide additional functionality to the phycobiliprotein pigments when it comes to multiplexing assays or to getting dyes into the cell.
Streptavidin-CryptoFluor Crimson is used to label biotin-conjugated (biotinylated) molecules and biotin derivatized surfaces in a wide variety of immunofluorescence applications such as immunochemistry, histochemistry and flow cytometry. Streptavidin-CryptoFluor Crimson may be used in the development of bioassay devices such as Lab-on-a-chip (LoC) systems and microarrays.
One vial contents 0.5 mg of conjugate and ~11 mg additives (see reconstitution)
Reconstitute to 250 μl with destilled or deionized water (the solution will consist of 100 mM sodium phosphate pH 7.2, 50 mM sucrose, 0.05% sodium azide, 150 mM Sodium chloride, 0.1 % BSA and 2 mg/ml pigment). Do not freeze, store at 4°C in the dark.
CryptoFluor is a trademark of Martek Biosciences Corp.