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18507

Sigma-Aldrich

Atto 565 maleimide

BioReagent, suitable for fluorescence

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Empirical Formula (Hill Notation):
C37H37ClN4O10
Molecular Weight:
733.16
MDL number:
NACRES:
NA.32

product line

BioReagent

Quality Level

Assay

>90.0% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
565 nm

fluorescence

λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0

λ

(ethanol with 0.1% trifluoroacetic acid)

UV absorption

λ: 560-566 nm Amax

suitability

corresponds for coupling to thiols
suitable for fluorescence

storage temp.

−20°C

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Atto 565 maleimide BioReagent, suitable for fluorescence

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Atto 565 maleimide

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Atto 655-maleimide

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Atto 655 NHS ester

assay

>90.0% (HPLC)

assay

≥90% (coupling rate)

assay

≥85% (HPLC)

assay

≥90.0% (HPLC)

form

powder

form

powder

form

powder

form

powder

fluorescence

λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 663 nm; λem 684 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

fluorescence

-

suitability

corresponds for coupling to thiols, suitable for fluorescence

suitability

-

suitability

-

suitability

suitable for coupling to amines, suitable for fluorescence

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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