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19123

Sigma-Aldrich

Nile Red

BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

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Synonym(s):
Nile Blue A Oxazone
Empirical Formula (Hill Notation):
C20H18N2O2
CAS Number:
Molecular Weight:
318.37
Beilstein:
279110
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

product line

BioReagent

Assay

≥97.0% (HPLC)

form

solid

mp

203-205 °C (lit.)
205-215 °C

λmax

553 nm

fluorescence

λex 530 nm; λem 635 nm in methanol

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34

InChI

1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3

InChI key

VOFUROIFQGPCGE-UHFFFAOYSA-N

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This Item
N301372485N5632
Nile Red BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Sigma-Aldrich

19123

Nile Red

Essential+ Grade
Nile Red Technical grade

Sigma-Aldrich

N3013

Nile Red

-
Nile Red for microscopy

Sigma-Aldrich

72485

Nile Red

-
Nile Blue A Dye content ≥75 %

Sigma-Aldrich

N5632

Nile Blue A

-
assay

≥97.0% (HPLC)

assay

-

assay

-

assay

-

form

solid

form

powder

form

crystals

form

powder

mp

203-205 °C (lit.), 205-215 °C

mp

203-205 °C (lit.)

mp

203-205 °C (lit.)

mp

>300 °C (dec.) (lit.)

λmax

553 nm

λmax

553 nm

λmax

553 nm

λmax

-

fluorescence

λex 530 nm; λem 635 nm in methanol

fluorescence

-

fluorescence

-

fluorescence

-

General description

Nile Red is an uncharged hydrophobic molecule and a benzophenoxazone dye. It functions as a fluorescent probe for intracellular lipids and hydrophobic domains of proteins. This dye is fluorescent in all organic solvents. Its fluorescence colors range from golden yellow to deep red.

Application

Nile Red has been used for the staining of intracellular lipid droplets and neutral lipids.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces
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