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19899

Sigma-Aldrich

CHAPS 100 mM solution

Synonym(s):

CHAPS solution

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About This Item

Empirical Formula (Hill Notation):
C32H58N2O7S
CAS Number:
Molecular Weight:
614.88
MDL number:
UNSPSC Code:
12161902
PubChem Substance ID:
NACRES:
NA.25

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description

zwitterionic

form

liquid

mol wt

614.88 g/mol

concentration

100 mM in H2O

technique(s)

protein quantification: suitable

CMC

8 mM

transition temp

cloud point >100 °C

density

1.01 g/mL at 20 °C

storage temp.

2-8°C

SMILES string

C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1

InChI key

UMCMPZBLKLEWAF-RFCNGIAKSA-N

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Show Differences

1 of 4

This Item
CHAPS-RO3050-OP1.11662
CHAPS 98% (from N), suitable for dialysis, solubility: >50% (Aqueous solutions)

CHAPS-RO

CHAPS

CHAPS OmniPur® Grade, Zwitterionic detergent

3050-OP

CHAPS

form

liquid

form

crystals

form

crystalline powder

form

solid

technique(s)

protein quantification: suitable

technique(s)

dialysis: suitable

technique(s)

-

technique(s)

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

10-30°C

storage temp.

2-8°C

CMC

8 mM

CMC

4 mM, 6 mM (20-25°C)

CMC

6 mM (20-25°C)

CMC

6 mM (20-25°C)

mol wt

614.88 g/mol

mol wt

micellar avg mol wt 6150, 614.88 g/mol (anhydrous basis)

mol wt

micellar avg mol wt 6150

mol wt

micellar avg mol wt 6150

density

1.01 g/mL at 20 °C

density

-

density

-

density

-

General description

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) is a biocompatible surfactant. It is a derivative of cholic acid comprising a hydrophobic cholesterol-like skeleton possessing three hydroxyl groups and a zwitterionic aliphatic tail.[1]

Application

CHAPS 100 mM solution has been used as a component of lysis buffer for the extraction of proteins from goat endometrial epithelial cells (EECs)[2] and liver tissue.[3]

Biochem/physiol Actions

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) exhibits the properties of both sulfobetaine-type surfactants and bile salts. It is involved in the protein extraction process for proteomic analysis and reconstitution of membrane proteins into lipid vesicles (proteoliposomes). CHAPS is broadly used in biochemistry for membrane protein and receptor isolation and purification. It is conveniently used in pharmaceuticals and medicines due to its biocompatibility, noncharged character, nondenaturing, and disaggregating properties.[1]

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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