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21878

Sigma-Aldrich

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester

BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

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Synonym(s):
5(6)-FAM SE, FLUOS
Empirical Formula (Hill Notation):
C25H15NO9
CAS Number:
117548-22-8
Molecular Weight:
473.39
MDL number:
MFCD04119713
PubChem Substance ID:
57647947
NACRES:
NA.32

product line

BioReagent

Assay

≥80% (HPLC)

form

powder

solubility

DMF: soluble
DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm in DMF

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

OC(=O)c1ccc(cc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O.OC(=O)c6cccc(C(=O)ON7C(=O)CCC7=O)c6C8=C9C=CC(=O)C=C9Oc%10cc(O)ccc8%10

InChI

1S/2C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)18-9-12(1-4-15(18)24(31)32)25(33)35-26-21(29)7-8-22(26)30;27-12-4-6-14-18(10-12)34-19-11-13(28)5-7-15(19)22(14)23-16(24(31)32)2-1-3-17(23)25(33)35-26-20(29)8-9-21(26)30/h1-6,9-11,27H,7-8H2,(H,31,32);1-7,10-11,27H,8-9H2,(H,31,32)

InChI key

YVTPVJDECOUTJW-UHFFFAOYSA-N

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This Item
469359284621955
5(6)-Carboxyfluorescein N-hydroxysuccinimide ester BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

Sigma-Aldrich

21878

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester

6-[Fluorescein-5(6)-carboxamido]hexanoic acid suitable for fluorescence, ≥90% (HPCE)

Sigma-Aldrich

46935

6-[Fluorescein-5(6)-carboxamido]hexanoic acid

5-Carboxyfluorescein N-succinimidyl ester BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sigma-Aldrich

92846

5-Carboxyfluorescein N-succinimidyl ester

5(6)-Carboxytetramethylrhodamine N-succinimidyl ester BioReagent, suitable for fluorescence, ≥70% (coupling to amines)

Sigma-Aldrich

21955

5(6)-Carboxytetramethylrhodamine N-succinimidyl ester

assay

≥80% (HPLC)

assay

≥90% (HPCE)

assay

≥90% (HPLC)

assay

≥70% (coupling to amines)

form

powder

form

solid

form

powder

form

powder

solubility

DMF: soluble, DMSO: soluble

solubility

DMF: soluble, DMSO: soluble

solubility

-

solubility

DMF: soluble, acetonitrile: soluble, methanol: soluble

fluorescence

λex 492 nm; λem 517 nm in DMF

fluorescence

λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0

fluorescence

λex 492 nm; λem 520 nm in 0.1 M Tris pH 8.0

fluorescence

λex 543 nm; λem 576 nm in methanol, λex 554 nm; λem 584 nm in 0.1 M phosphate pH 8.0

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

Application

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5(6)-FAM SE, FLUOS) may be used to prepare fluorescent derivatives of proteins such as tubulin and for the preparation of a phenytoin tracer for the determination of this and other drugs in serum and plasma by fluorescence polarization immunoassay (FPIA) and laser-induced fluorescence detection.
Fluorescent label for primary and secondary amine groups on proteins and other biomolecules.
Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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