Based on the following study, which involved ROS analysis of cells seeded in 6-well plates, it may be possible to use 96-well plates: https://doi.org/10.1016/j.lfs.2018.08.056
21884
5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate
BioReagent, suitable for fluorescence, ≥85% (HPCE)
5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate
Synonym(s):
5&6DH-DA, CDFDA, Carboxy-DCFDA
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About This Item
Empirical Formula (Hill Notation):
C25H14Cl2O9
CAS Number:
Molecular Weight:
529.28
UNSPSC Code:
12352106
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
7505106
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InChI
1S/2C25H14Cl2O9/c1-10(28)33-21-8-19-15(6-17(21)26)25(14-4-3-12(23(30)31)5-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19;1-10(28)33-21-8-19-15(6-17(21)26)25(14-5-12(23(30)31)3-4-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19/h2*3-9H,1-2H3,(H,30,31)
SMILES string
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5cc(ccc45)C(O)=O)c2cc1Cl.CC(=O)Oc6cc7Oc8cc(OC(C)=O)c(Cl)cc8C9(OC(=O)c%10ccc(cc9%10)C(O)=O)c7cc6Cl
InChI key
LZMPJBOMBCYYMP-UHFFFAOYSA-N
product line
BioReagent
assay
≥85% (HPCE)
mp
210 °C (dec.) (lit.)
solubility
DMF: soluble, DMSO: soluble, methanol: soluble
fluorescence
λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase)
suitability
suitable for fluorescence
storage temp.
−20°C
Quality Level
Related Categories
1 of 4
This Item | 21879 | 35845 | 35848 |
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| fluorescence λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase) | fluorescence λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase) | fluorescence λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase) | fluorescence λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0 |
| solubility DMF: soluble, methanol: soluble, DMSO: soluble | solubility DMSO: soluble | solubility DMSO: soluble | solubility - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
| assay ≥85% (HPCE) | assay ≥90.0% (HPLC) | assay ≥95% (HPLC) | assay ≥90% (T) |
General description
Application
5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate or Carboxy-DCFDA is used as a cell permeate probe to detect intracellular reactive oxygen species (ROS). [3] The anionic fluorescent dye, 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate is suitable for studying Multi-Drug Resistance (MDR) by target cells.[4]
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Analysis Note
Excmax: 504 nm (Lit.)
Other Notes
Intracellular esterase substrate with low sensitivity of fluorescence to intracellar pH[5]
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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O produto 21884-100MG pode ser utilizado para medir ROS em leitores de microplacas de 96 poços?
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