No direct reconstitution protocol is available for this product. Based on an online literature search, the CFSE dye, purchased as carboxyfluorescein diacetate succinimidyl ester (MW 557.47 g/mole), is typically provided in 25 mg vials. To prepare a final stock solution of 5 mM, dissolve the 25 mg in 8.96 mL DMSO. This solution can be stored as 50-100 μL aliquots at -20 °C for several months. It is important to avoid exposure to aqueous solutions during storage. Note that the CFSE dye is at a final concentration of 5 μM, however, the optimum concentration may have to be determined for each batch prepared, and then checked every 6 months to ensure it is effective.
21888
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester
BioReagent, suitable for fluorescence, ≥90% (HPLC)
Synonym(s):
CFSE
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About This Item
Empirical Formula (Hill Notation):
C29H19NO11
CAS Number:
Molecular Weight:
557.46
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352108
MDL number:
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InChI
1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3
SMILES string
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7
InChI key
LZFDYQHOQDFMOA-UHFFFAOYSA-N
Quality Level
description
suitable as fluorogenic substrate for esterases
product line
BioReagent
assay
≥90% (HPLC)
form
solid
mol wt
557.46 g/mol
color
almost white to yellow
mp
152-154 °C (lit.)
solubility
DMF: soluble, DMSO: soluble
fluorescence
λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)
suitability
suitable for fluorescence
storage temp.
−20°C
Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| fluorescence λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase) | fluorescence λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase) | fluorescence λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase) | fluorescence λex 492 nm; λem 517 nm in DMF |
| form solid | form - | form powder | form powder |
| solubility DMF: soluble, DMSO: soluble | solubility DMF: soluble, DMSO: soluble, methanol: soluble | solubility DMSO: soluble | solubility DMF: soluble, DMSO: soluble |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
General description
5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.
Application
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry.[1][2] Forms green fluorescent conjugates on deacetylation.
Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.
5-Carboxyfluorescein diacetate N-succinimidyl ester has been used:
- to label cells for flow cytometry
- in lymphocyte proliferation assay
- to determine the intracellular or intra-organellar pH in chloroplasts
- to label Leishmania amazonensis and Leishmania major
Biochem/physiol Actions
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
related product
Product No.
Description
Pricing
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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M Bronner-Fraser
The Journal of cell biology, 101(2), 610-617 (1985-08-01)
The possible role of a 140-kD cell surface complex in neural crest adhesion and migration was examined using a monoclonal antibody JG22, first described by Greve and Gottlieb (1982, J. Cell. Biochem. 18:221-229). The addition of JG22 to neural crest
WNT5A transforms intestinal CD8αα+ IELs into an unconventional phenotype with pro-inflammatory features
Zhao D, et al.
BMC Gastroenterology, 15(1), 173-173 (2015)
Autophagic induction greatly enhances Leishmania major intracellular survival compared to Leishmania amazonensis in CBA/j-infected macrophages
Dias BRS, et al.
Frontiers in Microbiology, 9 (2018)
A Reliable and Non-destructive Method for Monitoring the Stromal pH in Isolated Chloroplasts Using a Fluorescent pH Probe
Su PH and Lai YH
Frontiers in Plant Science, 8, 2079-2079 (2017)
Microbial Food Safety Along the Dairy Chain (2017)
Articles
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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Hi. I bought your CFSE. I want to ask how to use it? I follow one paper: doi: 10.1016/j.xcrm.2025.102027. They used CFSE in the final concentration of 0.5 uM for 3 mins at RT. Is this you suggested? And if yes, how to prepare 0.5 uM?
1 answer-
Helpful?
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What is the in vivo (mouse) half-life of CFDA-SE? What is the route of clearance (kidney, liver?)
1 answer-
The half-life is not determined for this product.
Please see the link below to a publication that may be helpful:
https://www.semanticscholar.org/paper/The-Use-of-Carboxyfluorescein-Diacetate-Ester-to-Quah-Parish/cae3435e063cb9f46cea4ddfc7c560f5821e19b3Please note that, unless otherwise stated, this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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What is the ratio between the (5) and (6) isomer of Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester?
1 answer-
Our Quality Control group does not routinely report the ratio; however, it is very likely that both isomers are present.
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At what concentration is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, soluble in DMSO?
1 answer-
We have found 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester to be soluble in DMSO at a concentration of 1 mg/mL.
Helpful?
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Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, fluorescent? Are conjugates of this compound fluorescent?
1 answer-
No. This compound and conjugates of this compound become fluorescent only when acted upon by an enzyme such as esterase. The enzyme removes the acetate groups.
Helpful?
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Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, light sensitive?
1 answer-
Yes. The powder and solutions (especially solutions) should be kept protected from ambient light whenever possible.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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