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21932

5(6)-Carboxynaphthofluorescein

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

Empirical Formula (Hill Notation):
C29H16O7
CAS Number:
128724-35-6
Molecular Weight:
476.43
MDL number:
MFCD01862976
UNSPSC Code:
12352108
PubChem Substance ID:
329752223
NACRES:
NA.32

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Product Name

5(6)-Carboxynaphthofluorescein, BioReagent, suitable for fluorescence, ≥90% (HPLC)

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc5cc(O)ccc5c4Oc6c3ccc7cc(O)ccc67.OC(=O)c8ccc9C(=O)OC%10(c%11ccc%12cc(O)ccc%12c%11Oc%13c%10ccc%14cc(O)ccc%13%14)c9c8

InChI

1S/2C29H16O7/c30-17-4-6-19-14(11-17)1-9-23-25(19)35-26-20-7-5-18(31)12-15(20)2-10-24(26)29(23)22-8-3-16(27(32)33)13-21(22)28(34)36-29;30-17-4-7-19-14(11-17)2-9-22-25(19)35-26-20-8-5-18(31)12-15(20)3-10-23(26)29(22)24-13-16(27(32)33)1-6-21(24)28(34)36-29/h2*1-13,30-31H,(H,32,33)

InChI key

ANUHNEJUELPITC-UHFFFAOYSA-N

product line

BioReagent

assay

≥90% (HPLC)

form

solid

pKa 

7.6

fluorescence

λex 512 nm; λem 567 nm (acid/neutral)
λex 598 nm; λem 668 nm (basic)

suitability

suitable for fluorescence

Quality Level

100

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469354694046950
5(6)-Carboxynaphthofluorescein BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sigma-Aldrich

21932

5(6)-Carboxynaphthofluorescein

6-[Fluorescein-5(6)-carboxamido]hexanoic acid suitable for fluorescence, ≥90% (HPCE)

Sigma-Aldrich

46935

6-[Fluorescein-5(6)-carboxamido]hexanoic acid

6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester suitable for fluorescence, ≥75% (HPLC)

Sigma-Aldrich

46940

6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester

Fluorescein 5(6)-isothiocyanate BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

Sigma-Aldrich

46950

Fluorescein 5(6)-isothiocyanate

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

fluorescence

λex 512 nm; λem 567 nm (acid/neutral), λex 598 nm; λem 668 nm (basic)

fluorescence

λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0

fluorescence

λex 490 nm; λem 514 nm (pH 8.2), λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0

fluorescence

λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0

form

solid

form

solid

form

solid

form

-

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

assay

≥90% (HPLC)

assay

≥90% (HPCE)

assay

≥75% (HPLC)

assay

≥90% (HPLC)

product line

BioReagent

product line

-

product line

-

product line

BioReagent

Application

5(6)-Carboxynaphthofluorescein (CNF) may be used as an intravesicular pH-sensitive probe for the detection of activity, selectivity and cooperativity of ion channels such as gramicidin A.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK9395
BCCD2352
BCBW8708
BCBW3158
BCBT9341

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A Song et al.
Analytical chemistry, 69(5), 863-867 (1997-03-01)
A fast and durable ratiometric pH microoptode that is highly accurate, precise, sensitive, reversible, and reproducible over the physiological ranges of pH, ionic strength, and temperature has been developed. The sensing site consists of 5 (and 6)-carboxynaphthofluorescein (CNF) entrapped in
D A Jankowska et al.
Biosensors & bioelectronics, 87, 312-319 (2016-08-31)
Aging population and longer life expectancy are the main reasons for an increasing number of patients with wound problems. Although the interest in wound care increases continuously, wound management still remains a challenge mainly due to the higher occurrence of
Shannon G Klein et al.
Global change biology, 23(9), 3690-3703 (2017-04-09)
Anthropogenic nutrient inputs enhance microbial respiration within many coastal ecosystems, driving concurrent hypoxia and acidification. During photosynthesis, Symbiodinium spp., the microalgal endosymbionts of cnidarians and other marine phyla, produce O
M Ramanathan et al.
Biosensors & bioelectronics, 22(12), 3001-3007 (2007-02-03)
The aim of the present work is to develop an evanescence wave array biosensor exploiting the "kinetic" approach of enzymatic reaction and further detection of the reaction products via pH sensitive fluorophore reporter. To demonstrate the feasibility of this approach
Sara M Butterfield et al.
Organic & biomolecular chemistry, 7(9), 1784-1792 (2009-07-11)
The activity of synthetic pores, ion channels, transporters and carriers is usually determined with fluorescent probes in vesicles or by conductance measurements in planar lipid bilayers. Elaborating on more colorful alternatives, we here introduce 5(6)-carboxynaphthofluorescein (CNF) as an intravesicular pH
View All Related Papers

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