MilliporeSigma
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24571C

SAFC

EX-CELL® 293 Serum-Free Medium for HEK 293 Cells

Animal-protein free, with L-glutamine, without sodium bicarbonate, dry powder, suitable for cell culture

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MDL number:
NACRES:
NA.71

Quality Level

form

dry powder

technique(s)

cell culture | mammalian: suitable (suspension)

shipped in

ambient

storage temp.

2-8°C

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This Item
14571C24326C24660C
technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

500

General description

EX-CELLTM 293 is an animal-protein free, serum-free dry powder medium developed for the long-term growth of Human Embryonic Kidney 293 (HEK 293) and related cells. The cells, in a suspension culture, can be subcultured directly into EX-CELLTM 293 from serum-supplemented media with little or no adaptation. Suspension cultures can be maintained without refeeding for about 10 days and can be carried for more than 20 passages with no loss of viability.

Application

EX-CELL 293 is an animal-protein free, serum-free medium developed for the long-term growth of Human Embryonic Kidney 293 (HEK 293) and related cells. The cells, in a suspension culture, can be subcultured directly into EX-CELL 293 from serum-supplemented media with little or no adaptation. Suspension cultures can be maintained, without refeeding, for about 10 days and can be carried for more that 20 passages with no loss of viability.

Quantity

Formulated to contain 21.6 grams of powder per liter of medium.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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G Schoofs et al.
Cytotechnology, 28(1-3), 81-89 (2008-11-13)
We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum
Pratik Jaluria et al.
Biotechnology and bioengineering, 99(6), 1443-1452 (2007-11-21)
Animal-derived serum is an essential media supplement for mammalian cells in cell culture. For a number of reasons including cost, regulatory concerns, lot inconsistency, potential contamination with adventitious agents, and down-stream processing it is desirable to eliminate the use of
J B Kaufman et al.
Cytotechnology, 33(1-3), 3-11 (2008-11-13)
The extracellular domain of human parathyroid Ca(2+) receptor was needed in order to study itsstructure and clinical application. The Ca(2+)receptor is a unique member of the G protein-coupledreceptor super-family, expressed in parathyroid andkidney cells where it has been shown to

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