Compare the use of Proteinase K for 5 and 10 minutes, which should be sufficient. There's no need to run HIER or antigen retrieval, as the Enzyme should effectively open the epitope sites for this antibody.
248M-9
Ep-CAM/Epithelial Specific Antigen (Ber-EP4) Mouse Monoclonal Antibody
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.1 mL | Check Cart for Availability | $176.00 | |
| 1 mL | Check Cart for Availability | $190.00 | |
| 0.5 mL | Check Cart for Availability | $451.00 | |
| 7 mL | Check Cart for Availability | $563.00 | |
| 1 mL | Check Cart for Availability | $730.00 |
About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
Ber-EP4, monoclonal
Application:
IHC (p)
Citations:
7
biological source
mouse
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
Ber-EP4, monoclonal
description
For In Vitro Diagnostic Use in Select Regions (See Chart)
form
buffered aqueous solution
species reactivity
human
packaging
vial of 0.1 mL concentrate (248M-94), vial of 0.5 mL concentrate (248M-95), bottle of 1.0 mL predilute (248M-97), vial of 1.0 mL concentrate (248M-96), bottle of 7.0 mL predilute (248M-98)
manufacturer/tradename
Cell Marque®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200
isotype
IgG1κ
control
colon carcinoma
shipped in
wet ice
storage temp.
2-8°C
visualization
cytoplasmic
Gene Information
human ... EPCAM(4072)
General description
Epithelial cell adhesion molecule (Ep-CAM) is a transmembrane glycoprotein localized on the membrane of cells in most epithelial tissues. Immunoreactivity with the antibody to E-CAM has been seen in the majority of epithelial neoplasms, whereas most non-epithelial neoplasms do not show Ep-CAM expression.[1] Ep-CAM is not expressed in mesothelial cells, hepatocytes, and lymphocytes.[1] In conjunction with other markers, Ep-CAM can be used as an aid in determining the epithelial origin of neoplasms such as lung adenocarcinoma.[1] [2]
Physical form
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Preparation Note
Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.
Analysis Note
 IVD |  IVD |  IVD |  RUO |
Other Notes
Ep-CAM Positive Control Slides, Product No. 248S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
For Technical Service please contact: 800-665-7284 or email: [email protected]
Legal Information
Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
This Item | |||
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| antibody form culture supernatant | antibody form culture supernatant | antibody form culture supernatant | antibody form purified immunoglobulin |
| clone Ber-EP4, monoclonal | clone MOC-31, monoclonal | clone Ber-EP4, monoclonal | clone VU-1D9, monoclonal |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form - |
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Related Content
Diagnostic immunohistochemistry overview highlights its importance in cancer diagnosis and detecting infectious diseases in modern clinical pathology.
Una visión general de la inmunohistoquímica diagnóstica destaca su importancia en el diagnóstico del cáncer y la detección de enfermedades infecciosas en la anatomopatología moderna.
Nelson G Ordóñez
Advances in anatomic pathology, 13(1), 16-25 (2006-02-08)
At present, a large number of immunohistochemical markers that can be used in the differential diagnosis between epithelioid peritoneal mesotheliomas and serous carcinomas are available. However, great differences of opinion exist regarding the individual value of some of these markers.
U Latza et al.
Journal of clinical pathology, 43(3), 213-219 (1990-03-01)
A new monoclonal antibody, Ber-EP4, directed against a partially formol resistant epitope on the protein moiety of two 34 kilodalton and 39 kilodalton glycopolypeptides on human epithelial cells is described. Immunostaining of a wide range of normal and neoplastic human
Nelson G Ordóñez
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 19(3), 417-428 (2006-01-18)
As both mesotheliomas and squamous carcinomas can present a wide variety of morphological patterns, they can on occasion be confused. Recently, some groups of investigators have called attention to the difficulties that sometimes exist in distinguishing between these malignancies and
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 248M-95 | 0841683130239 |
| 248M-97 | 0841683130253 |
| 248M-94 | 0841683130222 |
| 248M-96 | 0841683130246 |
| 248M-98 | 0841683130260 |
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how to use Proteinase K for the Ber-ep4?
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