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26745

Sigma-Aldrich

Cholesterol Esterase from porcine pancreas

lyophilized, powder, white, ~35 U/mg

Synonym(s):
Cholesterol Esterase from hog pancreas, Sterol-ester acylhydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

form

powder

Quality Level

quality

lyophilized

specific activity

~35 U/mg

mol wt

Mr ~440000

color

white

storage temp.

−20°C

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General description

Cholesterol Esterase is a serine hydrolase released from the exocrine pancreas. It belongs to the α/β hydrolase family of enzymes.

Application

Cholesterol Esterase from porcine pancreas has been used:
  • in an in vitro simulated digestion model to improve the hydrolysis of carotenoid esters in the intestinal phase
  • to analyze the bioavailability of phytosterol and cholesterol in the aqueous micellar phase of an in vitro simulated digestion model
  • as a standard in enzyme activity assay

Biochem/physiol Actions

For the hydrolysis of cholesterol esters in the determination of total cholesterol in serum or plasma; Application in (selective) hydrolysis/condensation of carboxylic ester bonds
Cholesterol esterase exhibits broad substrate specificity and regulates the bile salt-mediated hydrolysis of dietary cholesteryl esters. It participates in the hydrolysis of triglycerides and phospholipids which are essential for normal cholesterol absorption. Cholesterol esterase plays a key role in transporting free cholesterol to enterocytes. Loss of enzyme function reduces the intestinal absorption of dietary cholesteryl esters.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol cholesterol per minute at pH 7.0 and 37°C (cholesterol acetate as substrate)

Other Notes

Sales restrictions may apply

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Baojian Li et al.
European journal of medicinal chemistry, 45(5), 1955-1963 (2010-02-13)
Due to the importance of pancreatic cholesterol esterase (CEase) as a potential target in atherosclerosis and for the development of hypocholesterolemic agents, there are increasing interests in designing and synthesizing CEase inhibitors. In the present study, we prepared forty-five isocoumarin
R.J. Kazlauskas
Journal of the American Chemical Society, 111, 4953-4953 (1989)
J. Siedel et al.
Methods of Enzymatic Analysis, 8, 139-139 (1985)
Justin J Heynekamp et al.
Bioorganic & medicinal chemistry, 16(9), 5285-5294 (2008-03-21)
Pancreatic cholesterol esterase (CEase), which is secreted from the exocrine pancreas, is a serine hydrolase that aids in the bile salt-dependent hydrolysis of dietary cholesteryl esters and contributes to the hydrolysis of triglycerides and phospholipids. Additional roles for CEase in
Ömer Şahin et al.
Journal of biomolecular structure & dynamics, 1-15 (2021-01-23)
In this work, Combining coumarin and thiazole with 3-tertiary butyl salicylaldehyde into in a single molecule, new Schiff base (CTS), and its metal complexes with palladium and platinum were synthesized and characterized by using well-known spectroscopic techniques such as 1H-NMR

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