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28562

Sigma-Aldrich

Atto 488 maleimide

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):
Atto 488
MDL number:
NACRES:
NA.32

product line

BioReagent

Quality Level

assay

≥90% (HPLC)
≥90% (degree of coupling )

manufacturer/tradename

ATTO-TEC GmbH

λ

in methanol: water (1:1) (with 0.1% perchloric acid)

UV absorption

λ: 501-506 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 488 Maleimide is a hydrophilic fluorescent label with high molecular absorption (90.000) and quantum yield (0.80). The fluorescence activity is excited at a maximum of 501nm, and the maximum emission is 523nm. Atto 488 Maleimide is optimized for argon laser excitation and characterized by high photostability. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Post coupling to a substrate, the label carries a net electrical charge of -1.

Application

Atto 488 Maleimide is used for labelling amines, which usually requires a higher pH than the reaction of maleimides with thiols. It is a well-suited fluorescent label for thiol groups.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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