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28562

Atto 488 maleimide

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):

Atto 488

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1 MG

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About This Item

UNSPSC Code:
12352111
NACRES:
NA.32
MDL number:
MFCD07784936

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product line

BioReagent

assay

≥90% (HPLC), ≥90% (degree of coupling )

manufacturer/tradename

ATTO-TEC GmbH

λ

in methanol: water (1:1) (with 0.1% perchloric acid)

UV absorption

λ: 501-506 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

Quality Level

100

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This Item
410514169874417
Sigma-Aldrich

Sigma-Aldrich

28562

Atto 488 maleimide

Sigma-Aldrich

Sigma-Aldrich

41051

Atto 488

Sigma-Aldrich

Sigma-Aldrich

41698

Atto 488 NHS ester

Sigma-Aldrich

Sigma-Aldrich

74417

Atto 488 amine

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

assay

≥90% (HPLC), ≥90% (degree of coupling )

assay

≥90% (HPLC)

assay

≥90% (HPLC), ≥90% (degree of coupling)

assay

≥90% (HPLC)

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

BioReagent

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

General description

Atto 488 Maleimide is a hydrophilic fluorescent label with high molecular absorption (90.000) and quantum yield (0.80). The fluorescence activity is excited at a maximum of 501nm, and the maximum emission is 523nm. Atto 488 Maleimide is optimized for argon laser excitation and characterized by high photostability. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Post coupling to a substrate, the label carries a net electrical charge of -1.

Application

Atto 488 Maleimide is used for labelling amines, which usually requires a higher pH than the reaction of maleimides with thiols. It is a well-suited fluorescent label for thiol groups.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN9635
BCCN8588
BCCN7141
BCCN3499
BCCN2270

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Analysis of fluorescent nanostructures in biological systems by means of spectral position determination microscopy (SPDM).
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Dynamic reorganization of the actomyosin cytoskeleton allows fast modulation of the cell surface, which is vital for many cellular functions. Myosin-II motors generate the forces required for this remodeling by imparting contractility to actin networks. However, myosin-II activity might also
Jeanne C Stachowiak et al.
Nature cell biology, 14(9), 944-949 (2012-08-21)
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Markus Hirsch et al.
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Investigations into the fate of small interfering RNA (siRNA) after transfection may unravel new ways to improve RNA interference (RNAi) efficiency. Because intracellular degradation of RNA may prevent reliable observation of fluorescence-labeled siRNA, new tools for fluorescence microscopy are warranted
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