28562

Atto 488 maleimide

BioReagent, suitable for fluorescence, ≥90% (HPLC)

• Synonym(s): Atto 488
• MDL number: MFCD07784936
• NACRES: NA.32

Properties

• product line: BioReagent
• Assay: ≥90% (HPLC); ≥90% (degree of coupling )
• manufacturer/tradename: ATTO-TEC GmbH
• λ: in methanol: water (1:1) (with 0.1% perchloric acid)
• UV absorption: λ: 501-506 nm A_max
• suitability: suitable for fluorescence
• storage temp.: −20°C

Description

General description

Atto 488 Maleimide is a hydrophilic fluorescent label with high molecular absorption (90.000) and quantum yield (0.80). The fluorescence activity is excited at a maximum of 501nm, and the maximum emission is 523nm. Atto 488 Maleimide is optimized for argon laser excitation and characterized by high photostability. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Post coupling to a substrate, the label carries a net electrical charge of -1.

Application

Atto 488 Maleimide is used for labelling amines, which usually requires a higher pH than the reaction of maleimides with thiols. It is a well-suited fluorescent label for thiol groups.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves