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306-05A

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Human Cardiac Fibroblasts: HCF, adult

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Synonym(s):
HCF cells
NACRES:
NA.81

biological source

human heart (normal adult)

Quality Level

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

adherent

karyotype

2n = 46

morphology

fibroblast

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

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This Item
306-05F506-05AC-12375
Human Cardiac Fibroblasts (HCF) 500,000 cryopreserved cells

C-12375

Human Cardiac Fibroblasts (HCF)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cardiovascular diseases

relevant disease(s)

cardiovascular diseases

relevant disease(s)

asthma; tuberculosis; cardiovascular diseases; cancer

relevant disease(s)

cardiovascular diseases

morphology

fibroblast

morphology

fibroblast

morphology

fibroblast

morphology

(fibroblast)

biological source

human heart (normal adult)

biological source

human heart (normal)

biological source

human lung (normal parenchyma)

biological source

human heart (ventricles)

karyotype

2n = 46

karyotype

2n = 46

karyotype

2n = 46

karyotype

-

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cardiac fibroblasts are the most prevalent cell type in the heart, making up 60-70 % of all cells. HCF from Cell Applications, Inc. provide an excellent model system to study many aspects of human heart function and pathophysiology.

HCF has been utilized in a number of research publications, for example to:

  • Determine that electrical coupling between cardiomyocytes and fibroblasts is mediated by large-conductance Ca2+-activated K+ channels that can be stimulated by estrogen receptor agonists (Wang, 2006, 2007); and show that antimitogenic effects of estradiol on HCF growth are mediated by cytochromes 1A1/1B1-and catechol-O-methyltransferase-derived metabolites (Dubey, 2005)
  • Show that in response to mechanical stretch, cardiac fibroblasts release TGF-b which causes trombomodulin downregulation, increasing the risk of thromboembolic events (Kapur, 2007) and also induces cardiac fibroblast differentiation into myofibroblasts via increased Smad2 and ERK1/2 phosphorylation, that could be stimulated by endothelin-1 and inhibited by Ac-SDKP (Peng, 2010)
  • Demonstrate that activation of G protein-coupled receptor kinase-2 (GRK2) prevents normal regulation of collagen synthesis in cardiac fibroblasts mimicking heart failure phenotype (D’Souza, 2011); identify FGF2 signaling pathway as potential target for modulating apoptosis in cardiac pathology (Ma, 2011) and investigate the roles of scleraxis (Bagchi, 2012) and AMPKa1 (Noppe, 2014) in scar formation following myocardial infarction
  • Show that the KATP channel opener KMUP-3 preserved cardiac function after myocardial infarction by enhancing the expression of NO synthase and restoring MMP-9/TIMP-1 balance (Liu, 2011)

HCF were also shown to express delayed rectifier IK, Ito, Ca2+-activated K+ current (BKCa), inward-rectifier (Kir-type), and swelling-induced Cl- current (ICl.vol) channels (Yue, 2013).

Cell Line Origin

Heart

Application

human heart function, neointimal formation, vascular remodeling, myocardial repair, extracellular maintenance, signaling, toxicology, gene expression, structural support for cardiomycytes, synthesis of extracellular matrix, growth factors and cytokines

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 8 doublings

Subculture Routine

Please refer to the HCF Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Li Qiao et al.
The Journal of clinical investigation, 129(6), 2237-2250 (2019-04-30)
Exosomes, as functional paracrine units of therapeutic cells, can partially reproduce the reparative properties of their parental cells. The constitution of exosomes, as well as their biological activity, largely depends on the cells that secrete them. We isolated exosomes from

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