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30674

Sigma-Aldrich

Atto 700

BioReagent, suitable for fluorescence, ≥90.0% (HPCE)

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MDL number:
MFCD06798563
NACRES:
NA.32

product line

BioReagent

Quality Level

100

Assay

≥90.0% (HPCE)

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
700 nm

fluorescence

λex 681 nm; λem 714 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid: 692 nm ± 3 nm)

suitability

suitable for fluorescence

storage temp.

−20°C

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Atto 700

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assay

≥90.0% (HPCE)

assay

≥90% (HPLC)

assay

≥90.0% (HPLC)

assay

≥90.0% (HPCE)

fluorescence

λex 681 nm; λem 714 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4

fluorescence

λex 556 nm; λem 578.0 nm±5.0 nm in ethanol

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

General description

Atto 700 is a new label with high molecular absorption (1.2 x 105) and quantum yield (0.25) as well as sufficient Stoke′s shift between excitation and emission maximum. Atto 700 is characterized by high photostability and thermal stability. It is also excellently soluble in water. Due to an insignificant triplet formation rate, it is well suited for single molecule detection applications.

Application

Atto 700 belongs to a new generation of fluorescent labels. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, excellent thermal and photo-stability, very good water solubility and very little triplet formation. Atto 700 is a zwitterionic dye with a net electrical charge of zero. The fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc.
Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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