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Giemsa Stain, Modified Solution

according to Giemsa

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Synonym(s):
Azure eosin methylene blue, Giemsa solution
CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
MA.02

form

liquid

Quality Level

quality

according to Giemsa

technique(s)

UV/Vis spectroscopy: suitable

pH

6.9

density

1.036 g/cm3 at 20 °C

suitability

passes test for UV spectrum

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

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This Item
G563748900GS128
Giemsa stain certified by the Biological Stain Commission

Sigma-Aldrich

G5637

Giemsa stain

Giemsa Stain, Modified

Sigma-Aldrich

GS128

Giemsa Stain, Modified

technique(s)

UV/Vis spectroscopy: suitable

technique(s)

-

technique(s)

microbe id | staining: suitable

technique(s)

-

pH

6.9

pH

-

pH

-

pH

6.75-6.95

density

1.036 g/cm3 at 20 °C

density

-

density

-

density

-

suitability

passes test for UV spectrum

suitability

-

suitability

-

suitability

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

hematology
histology

General description

Modified Giemsa stain means when the pH of the Giemsa stain is changed from the usual 6.8 to 9.0.

Application

Modified Giemsa stain may be used for the identification of each human chromosome by differentials staining.

Other Notes

Giemsa stain, modified, 0.4%, w/v, in buffered methanol solution, pH 6.8. Also contains stabilizers.

Principle

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

51.8 °F - closed cup

Flash Point(C)

11 °C - closed cup


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