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32884

Sigma-Aldrich

Giemsa Stain, Modified Solution

according to Giemsa

Synonym(s):
Azure eosin methylene blue, Giemsa solution
CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
MA.02

form

liquid

Quality Level

quality

according to Giemsa

technique(s)

UV/Vis spectroscopy: suitable

pH

6.9

density

1.036 g/cm3 at 20 °C

suitability

passes test for UV spectrum

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

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This Item
G450748900G1025
Giemsa stain technical grade, used as a blood stain

Sigma-Aldrich

G4507

Giemsa stain

form

liquid

form

powder

form

-

form

-

technique(s)

UV/Vis spectroscopy: suitable

technique(s)

-

technique(s)

microbe id | staining: suitable

technique(s)

cell culture | plant: suitable

pH

6.9

pH

-

pH

-

pH

-

density

1.036 g/cm3 at 20 °C

density

-

density

-

density

-

suitability

passes test for UV spectrum

suitability

-

suitability

-

suitability

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

agriculture

General description

Modified Giemsa stain means when the pH of the Giemsa stain is changed from the usual 6.8 to 9.0.

Application

Modified Giemsa stain may be used for the identification of each human chromosome by differentials staining.

Other Notes

Giemsa stain, modified, 0.4%, w/v, in buffered methanol solution, pH 6.8. Also contains stabilizers.

Principle

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

51.8 °F - closed cup

Flash Point(C)

11 °C - closed cup

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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