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371R-2

Sigma-Aldrich

SOX-2 (EP103) Rabbit Monoclonal Primary Antibody

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biological source

rabbit

Quality Level

100
500

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

EP103, monoclonal

description

For In Vitro Diagnostic Use in Select Regions

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (371R-24)
vial of 0.1 mL concentrate Research Use Only (371R-24-RUO)
vial of 0.5 mL concentrate (371R-25)
vial of 1.0 mL concentrate (371R-26)
vial of 1.0 mL concentrate Research Use Only (371R-26-RUO)
vial of 1.0 mL pre-dilute Research Use Only (371R-27-RUO)
vial of 1.0 mL pre-dilute ready-to-use (371R-27)
vial of 7.0 mL pre-dilute ready-to-use (371R-28)
vial of 7.0 mL pre-dilute ready-to-use Research Use Only (371R-28-RUO)

manufacturer/tradename

Cell Marque

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200 (concentrated)

isotype

IgG

control

squamous carcinoma (lung)

shipped in

wet ice

storage temp.

2-8°C

visualization

nuclear

Gene Information

human ... SOX2(6657)

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This Item
AC-0344AAC-0344332R-1
clone

EP103, monoclonal

clone

monoclonal

clone

monoclonal

clone

EP130, monoclonal

conjugate

unconjugated

conjugate

-

conjugate

-

conjugate

unconjugated

antibody form

culture supernatant

antibody form

Ig fraction of antiserum

antibody form

Ig fraction of antiserum

antibody form

culture supernatant

species reactivity

human

species reactivity

-

species reactivity

-

species reactivity

human

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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General description

The differentiation of seminomas from non-seminomatous germ cell tumors can be challenging, especially, if small biopsy specimens, necrotic tumors and metastatic tumors with artifacts are encountered. A subset of germ cell tumors may require immunohistochemistry (IHC) for classification owing to unusual morphologic features, such as diffuse growth of clear cells, and tumors with glandular and/or microcytic patterns. In the mixed germ cell tumor, one component is often intermingled intimately with others such as embryonal carcinoma versus yolk sac tumor, can be overlooked. IHC will identify such an area and allow for the identification of each component of the mixed tumor more accurately and documenting them in the pathology report is recommended by WHO. Current IHC studies have shown the combination of CD30/CD117 staining plays a good role in distinguishing between embryonal carcinoma and yolk sac tumor. However, a subset of tumors may not be distinguished by this combination. Also, the characteristic membranous pattern by antibodies to CD30 and CD117 for the interpretation of the diagnosis may not be evident in limited biopsy specimens. In this respect, transcription factors, such as SOX-2, are easier to interpret due to their distinct nuclear reaction. SOX-2 has been reported as a diagnostic marker for embryonal carcinoma. SOX-2 was expressed in intratubular embryonal carcinoma, pure embryonal carcinoma and in the embryonal carcinoma component of mixed germ cell tumor in all cases. But, SOX-2 expression has not been found in seminoma, yolk sac tumor, and choriocarcinoma in almost all cases. An inclusion of transcription factors, like SOX-2 and SOX-17, in a panel of IHC is useful for the classification of germ cell tumors: that is, SOX-2+/OCT3/4+/CD30+/CD117-/SOX17- for embryonal carcinoma; SOX-2-/OCT3/4+/CD30-/CD117+/SOX17+ for seminoma; SOX-2-/OCT3/4-/CD30-/CD117-/SOX17- for yolk sac tumor.

Quality


IVD

IVD

IVD

RUO

Linkage

SOX-2 Positive Control Slides, Product No. 371S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide.

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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I A Sesterhenn et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 10(1), 69-78 (1992-01-01)
The Testicular Cancer Intergroup Study (TCIS) was undertaken to evaluate the pathologic findings in early-stage testicular cancer as determined by central pathology review, to compare these findings with the interpretation by the contributing pathologists, and to make correlations with various
Sandro Santagata et al.
The American journal of surgical pathology, 31(6), 836-845 (2007-05-29)
The core embryonic stem cell transcription factors (TFs) OCT3/4 (OCT4), NANOG, and SOX2 have shared as well as nonoverlapping roles in stem cell growth and differentiation. These same TFs are also expressed in various types of human germ cell tumors
Tumors of the testis and paratesticular tissue
Woodward PJ et al.
Pathology and Genetics of Tumours of the Urinary System and Male Genital Organs, Pages 217-Pages 278 (2004)
J de Jong et al.
The Journal of pathology, 215(1), 21-30 (2008-03-19)
Combined action of SOX and POU families of transcription factors plays major roles in embryonic development. In embryonic stem cells, the combination of SOX2 and POU5F1 (OCT3/4) is essential for maintaining the undifferentiated state by activating pluripotency-linked genes, and inhibition
Robert E Emerson et al.
Seminars in diagnostic pathology, 22(1), 33-50 (2006-03-04)
Although most testicular and paratesticular tumors can be recognized by their light microscopic features, some raise significant differential diagnostic questions. Immunohistochemical staining has proved of significant value in this situation. There is still a role for the traditional markers, including

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