388M-1

Sigma-Aldrich

Nestin (10C2) Mouse Monoclonal Antibody

NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

culture supernatant

antibody product type

primary antibodies

clone

10C2, monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (388M-14)
vial of 0.5 mL concentrate (388M-15)
bottle of 1.0 mL predilute (388M-17)
vial of 1.0 mL concentrate (388M-16)
bottle of 7.0 mL predilute (388M-18)

manufacturer/tradename

Cell Marque

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100

isotype

IgG1

conjugate

unconjugated

control

tonsil

shipped in

wet ice

storage temp.

2-8°C

visualization

cytoplasmic

General description

Nestin is a class VI intermediate filament (IF) protein. It has been reported that nestin expression is significantly increased in melanoma and correlated with more advanced stages of the disease. An immunohistochemical analysis identified nestin-positive cells in 84% (35/42) of primary melanoma and 83% (10/12) of metastatic melanoma. Nestin immunoreactivity was also observed in melanoma cells in all (10/10) cases of HMB-45-negative amelanotic and melanotic, non-desmoplastic melanoma. Nestin expression has been reported in tumors of the CNS, including astrocytoma, ependymoma, oligodendroglioma, glioblastoma, and primitive neuroectodermal tumors. Nestin has been detected in human gliomas, including low and high grade, but its expression is observed more frequently in high grade than in low grade gliomas. Nestin overexpression is seen in carcinomas, including prostatic adenocarcinoma, pancreatic ductal carcinoma, thyroid carcinoma, and in mesenchymal tumors such as GIST and DFSP. Among the breast carcinoma subtypes, nestin is highly expressed in basal breast cancer subtype (ERα-/PR-/Her2-) but not in the Her2 subtype (ERα-/PR-/Her2+) or luminal epithelial phenotype (ERα+/PR+). Only cytoplasmic staining is considered positive, whereas any nuclear staining is considered as background artifact. In normal skin, nestin is observed in endothelial cells and the bulge area of hair follicles.

Quality


IVD

IVD

IVD

RUO

Linkage

Nestin Positive Control Slides, Product No. 388S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

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Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

Certificate of Analysis

Certificate of Origin

Svetlana Brychtova et al.
Journal of cutaneous pathology, 34(5), 370-375 (2007-04-24)
Nestin is one of the intermediate filaments that are expressed in proliferating neural progenitor cells during development of the central nervous system (CNS) and peripheral nervous system. Postnatal re-expression of the protein occurs mainly under pathological conditions, including injury and...
Toshiyuki Ishiwata et al.
World journal of gastroenterology, 17(4), 409-418 (2011-01-29)
Nestin is a class VI intermediate filament protein that was originally described as a neuronal stem cell marker during central nervous system (CNS) development, and is currently widely used in that capacity. Nestin is also expressed in non-neuronal immature or...
Hua Li et al.
Cancer research, 67(2), 501-510 (2007-01-20)
Transcriptional profiling has identified five breast cancer subtypes, of which the basal epithelial is most aggressive and correlates with poor prognosis. These tumors display a high degree of cellular heterogeneity and lack established molecular targets, such as estrogen receptor-alpha, progesterone...
Maho Kanoh et al.
The Journal of dermatology, 37(6), 505-511 (2010-06-12)
Nestin, a marker of neural stem cells, is expressed in the stem cells of the mouse hair follicle. The nestin-expressing hair follicle stem cells can differentiate into neurons, glia, keratocytes, smooth muscle cells and melanocytes in vitro. These pluripotent nestin-expressing...
Articles
Immunohistochemistry (IHC) techniques and applications have greatly improved, dermatopathology is still largely based on H&E stained slides.This paper outlines ways in which IHC antibodies can be utilized for dermatopathology.
Read More

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