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39294

Sigma-Aldrich

Fluo 3-AM solution

1 mM in DMSO, ≥99.0% (TLC)

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About This Item

Empirical Formula (Hill Notation):
C51H50Cl2N2O23
CAS Number:
121714-22-5
Molecular Weight:
1129.85
MDL number:
MFCD00083336
UNSPSC Code:
12171500
NACRES:
NA.32

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assay

≥99.0% (TLC)

form

liquid

concentration

1 mM in DMSO

fluorescence

λex 506 nm; λem 525.0 nm±5.0 nm in 0.1 M Tris pH 8.0, esterase

storage temp.

−20°C

SMILES string

Clc1[c](cc2[o]c3c(c(c2c1)c4cc(c(cc4)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOc5c(ccc(c5)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)cc(c(c3)OCOC(=O)C)Cl)=O

InChI

1S/C51H50Cl2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2

InChI key

PTPUOMXKXCCSEN-UHFFFAOYSA-N

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1 of 4

This Item
343242-M34490647989
Fluo 3-AM solution 1 mM in DMSO, ≥99.0% (TLC)

39294

Fluo 3-AM solution

FLUO 3/AM Membrane-permeable version of FLUO 3. Unlike all other Ca2+ indicators, FLUO 3/AM is non-fluorescent until it is hydrolyzed in the cell by cellular esterases. It is insensitive to Mg2+.

343242-M

FLUO 3/AM

FURA 2/AM in Solution

344906

FURA 2/AM in Solution

Fura 2-AM BioReagent, suitable for fluorescence, ≥95.0% (HPLC)

47989

Fura 2-AM

form

liquid

form

solid

form

liquid

form

solid

assay

≥99.0% (TLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥95.0% (HPLC)

concentration

1 mM in DMSO

concentration

-

concentration

-

concentration

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

fluorescence

λex 506 nm; λem 525.0 nm±5.0 nm in 0.1 M Tris pH 8.0, esterase

fluorescence

-

fluorescence

λex 335 nm (high), λex 362 nm (low), λem 505 nm (high), λem 512 nm (low)

fluorescence

λex 355 nm; λem 495 nm in 0.1 M Tris pH 8.0, esterase; 10 mM Ca2+

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Cell permeable fluorescent indicator of intracellular Ca2+; non-fluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca2+; lower binding affinity allows measurement of higher peaks of Ca2+ transients than with Fura 2.

Application

suitable as fluorescent Ca sensitive probe (after cleavage of the AM ester)

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

188.6 °F

flash_point_c

87 °C

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG6962
BCCB4646
BCBV3584
BCBP7162V
BCBP1596V

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