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Supelco

NTA - Atto 488

BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE)

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Synonym(s):
Atto 488 - Nitrilotriacetic acid, complexed to Ni2+ - ion, Nα,Nα-bis(carboxymethyl)-L-lysine, Nickel(II) complex
UNSPSC Code:
12171501
NACRES:
NA.32

product line

BioReagent

Quality Level

100

assay

≥80% (sum of isomers, HPCE)

form

solid

fluorescence

λex 501 nm; λem 523 nm in PBS with 0.08% sodium azide

suitability

suitable for fluorescence

detection method

fluorometric

storage temp.

−20°C

Related Categories

General description

find more information here

Application

for the fluorescence detection of HIS-tagged proteins

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

pictograms

Health hazardEnvironment
GHS08,GHS09

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1A - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 Inhalation

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nitrilotriacetic acid Sigma Grade, ≥99%

Sigma-Aldrich

N9877

Nitrilotriacetic acid

N-[Nα,Nα-Bis(carboxymethyl)-L-lysine]-12-mercaptododecanamide ≥90.0% (TLC)

Sigma-Aldrich

38152

N-[Nα,Nα-Bis(carboxymethyl)-L-lysine]-12-mercaptododecanamide

Sigma-Aldrich

Sigma-Aldrich

97875

Atto 647

Nα,Nα-Bis(carboxymethyl)-L-lysine trifluoroacetate salt ≥95% (TLC)

Sigma-Aldrich

C3205

Nα,Nα-Bis(carboxymethyl)-L-lysine trifluoroacetate salt

Sigma-Aldrich

Sigma-Aldrich

41698

Atto 488 NHS ester

Sigma-Aldrich

Sigma-Aldrich

74417

Atto 488 amine

The changing point-spread function. Single-molecule-based super-resolution imaging.
Horrocks, M. H.; Palayret, M.; Klenerman, D.; Lee, S. F.
Histochemistry and Cell Biology, 141(6), 577-585 (2014)
Verbesserte hochauflosende Mikroskopie mit Oxazinfarbstoffen in schwerem Wasser.
Lee, S. F.; Verolet, Q.; Furstenberg, A.
Angewandte Chemie (International Edition in English), 124(34), 9117-9120 (2013)
Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging.
van de Linde, S.; et al.
Photochemistry and Photobiology, 10(4), 499-506 (2011)
Intrinsically Resolution Enhancing Probes for Confocal Microscopy.
Vogelsang, J.; et al.
Nano Letters, 10(2), 672-679 (2010)
Dynamic Submicroscopic Signaling Zones Revealed by Pair Correlation Tracking and Localization Microscopy.
You, Ch.; et al.
Analytical Chemistry, 86(17), 8593-8602 (2014)
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Articles

Ni-NTA-Atto Conjugates

Ni-NTA-Atto conjugates provide specific and highly sensitive detection of His-tagged fusion proteins.

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