MilliporeSigma
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39625

Supelco

NTA - Atto 488

BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE)

Synonym(s):
Atto 488 - Nitrilotriacetic acid, complexed to Ni2+ - ion, Nα,Nα-bis(carboxymethyl)-L-lysine, Nickel(II) complex
NACRES:
NA.32

product line

BioReagent

Quality Level

Assay

≥80% (sum of isomers, HPCE)

form

solid

fluorescence

λex 501 nm; λem 523 nm in PBS with 0.08% sodium azide

suitability

suitable for fluorescence

detection method

fluorometric

storage temp.

−20°C

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NTA - Atto 488

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Sigma-Aldrich

Sigma-Aldrich

94875

Atto 680

assay

≥80% (sum of isomers, HPCE)

assay

≥80% (HPCE)

assay

≥80% (sum of isomers, HPCE)

assay

≥90% (HPLC)

form

solid

form

-

form

lumps

form

powder

fluorescence

λex 501 nm; λem 523 nm in PBS with 0.08% sodium azide

fluorescence

λex 647 nm; λem 669 nm in PBS

fluorescence

λex 554 nm; λem 576 nm in PBS with 0.08% sodium azide

fluorescence

λex 680 nm; λem 695 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

General description

find more information here

Application

for the fluorescence detection of HIS-tagged proteins

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Pictograms

Health hazardEnvironment

Signal Word

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1A - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 Inhalation

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Flexible Microdomain Specific Staining of Block Copolymers for 3D Optical Nanoscopy.
Ullal, Chaitanya K.; et al.
Macromolecules, 44(19), 7508-7510 (2011)
Investigating Cellular Structures at the Nanoscale with Organic Fluorophores.
van de Linde, S.; et al.
Chemistry and Biology, 20(1), 8-18 (2013)
The changing point-spread function. Single-molecule-based super-resolution imaging.
Horrocks, M. H.; Palayret, M.; Klenerman, D.; Lee, S. F.
Histochemistry and Cell Biology, 141(6), 577-585 (2014)
Intrinsically Resolution Enhancing Probes for Confocal Microscopy.
Vogelsang, J.; et al.
Nano Letters, 10(2), 672-679 (2010)
Verbesserte hochauflosende Mikroskopie mit Oxazinfarbstoffen in schwerem Wasser.
Lee, S. F.; Verolet, Q.; Furstenberg, A.
Angewandte Chemie (International Edition in English), 124(34), 9117-9120 (2013)

Articles

Ni-NTA-Atto Conjugates

Ni-NTA-Atto conjugates provide specific and highly sensitive detection of His-tagged fusion proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service