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MilliporeSigma

40692

Sigma-Aldrich

Atto 532 DOPE

suitable for fluorescence

Synonym(s):

1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 532

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$711.00

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

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assay

≥80.0% (HPCE)

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 537 nm; λem 559 nm±5 nm in ethanol

suitability

suitable for fluorescence

storage temp.

−20°C

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This Item
422089007751016
assay

≥80.0% (HPCE)

assay

≥90.0% (HPCE)

assay

≥90.0% (TLC)

assay

≥90% (TLC)

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

fluorescence

λex 537 nm; λem 559 nm±5 nm in ethanol

fluorescence

λex 556 nm; λem 578.0 nm±5.0 nm in ethanol

fluorescence

-

fluorescence

-

General description

Atto 532 is a fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photostability, and excellent water solubility. Thus Atto 532 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. The fluorescence is excited most efficiently in the range 515 - 545 nm.
A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd:YAG laser.

Atto-Dye Labeled Phospholipids
Sigma-Aldrich offers a variety of glycero-phospholipids carrying one or two fatty acid groups (lipophilic groups) and a phosphate ester residue (hydrophilic group). They are labeled at the hydrophilic head group. After incorporation of the phospholipid into a membrane the fluorophore is located at the water/lipid interface of the membrane. We currently provide 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), palmitoyl-sn-glycero-phosphoethanolamine (PPE), and 1,2-dimyristoyl-sn-glycero-3-phospho-ethanolamine (DMPE) labeled with Atto-dyes.

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Technical Review. Types of Imaging-Direct STORM.
Jensen, E.; Crossman, D. J.
The Anatomical Record, 297(12), 2227-2231 (2014)
Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins.
Hofmann, M.; et al.
Proceedings of the National Academy of Sciences of the USA, 102(49), 17565?17569-17565?17569 (2005)
Fluoreszierende Rhodamine und fluorogene Carbopyronine fur die STED-Mikroskopie lebender Zellen.
Butkevich, Alexey N.; et al.
Angewandte Chemie (International Edition in English), 128(10), 3350-3355 (2016)
Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission.
Ge, J.; Kuang, C.; Lee, SS.; Kao, FJ
Optics Express, 20(27), 28216-28216 (2012)
Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature.
Eggeling, C.; et al.
Nature, 457, 1159-1162 (2009)

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