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MilliporeSigma

40709

Atto 590-Streptavidin

BioReagent, suitable for fluorescence

Synonym(s):

Streptavidin-Atto 590

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1 MG

$443.00

$443.00

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32
MDL number:
MFCD04040679

Key Documents

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Product Name

Atto 590-Streptavidin, BioReagent, suitable for fluorescence

product line

BioReagent

form

powder

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 590 nm; λem 619 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

Quality Level

100

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This Item
166309640456304
Sigma-Aldrich

Sigma-Aldrich

40709

Atto 590-Streptavidin

Sigma-Aldrich

Sigma-Aldrich

16630

Atto 680-Streptavidin

SAFC

SAFC

96404

Atto 550-Streptavidin

Sigma-Aldrich

Sigma-Aldrich

56304

Atto 565-Streptavidin

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

fluorescence

λex 590 nm; λem 619 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

λex 554 nm; λem 576 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 565 nm; λem 590 nm in 0.1 M phosphate pH 7.0

form

powder

form

powder

form

solid

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

BioReagent

Analysis Note

free of unconjugated dye

Application

Atto 590 is a new label with high molecular absorption (120.000) and quantum yield (0.80) as well as sufficient stoke′s shift (excitation maximum 594 nm, emission maximum 624 nm). Atto 590 has been coupled to streptavidin to enable all kinds of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays. Streptavidin conjugation of Atto 590 does not significantly change the labels spectral data regarding excitation and emission maxima. Given the optimized Dye/Protein-ratio molar absorbance and quantum yields remain high within application. Conjugates of our Atto dyes generally do not show strong fluorescence in unbound state, but fluorescence is boosted again as soon as they are bound to biotin. This may help overcome problems by unspecific binding which may sometimes appear with streptavidin based assays in special cases.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN2274
BCCK6831
BCCJ9339
BCCJ0118
BCCB2827

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Mariela M Marani et al.
Journal of combinatorial chemistry, 11(1), 146-150 (2008-12-17)
To screen one-bead-one-compound (OBOC) combinatorial libraries, tens of thousands to millions of compound beads are first mixed with a target molecule. The beads that interact with this molecule are then identified and isolated for compound structure determination. Here we describe
Ningtao Li et al.
Frontiers in endocrinology, 11, 545973-545973 (2020-10-27)
Testosterone (T), predominantly acting through its derivative 17β-estradiol (E2), regulates the brain's sexual differentiation in rodents during the perinatal sensitive period, which mirrors the window of vulnerability to the adverse effects of general anesthetics. The mechanisms of anesthesia's adverse effects
Andrea D Lehmann et al.
Small (Weinheim an der Bergstrasse, Germany), 6(6), 753-762 (2010-03-06)
Iron-platinum nanoparticles embedded in a poly(methacrylic acid) (PMA) polymer shell and fluorescently labeled with the dye ATTO 590 (FePt-PMA-ATTO-2%) are investigated in terms of their intracellular localization in lung cells and potential to induce a proinflammatory response dependent on concentration
Ronit Freeman et al.
Nano letters, 10(6), 2192-2196 (2010-05-21)
Semiconductor quantum dots (QDs) are used for the optical analysis of casein kinase (CK2) or the hydrolytic activity of alkaline phosphatase (ALP). Two schemes for the analysis of CK2 by a FRET-based mechanism are described. One approach involves the CK2-catalyzed
Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature.
Eggeling, C.; et al.
Nature, 457, 1159-1162 (2009)
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