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  • 40709
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40709

Sigma-Aldrich

Atto 590-Streptavidin

BioReagent, suitable for fluorescence

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Synonym(s):
Streptavidin-Atto 590
MDL number:
MFCD04040679
NACRES:
NA.32

product line

BioReagent

Quality Level

100

form

powder

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 590 nm; λem 619 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

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product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

BioReagent

form

powder

form

solid

form

powder

form

solid

fluorescence

λex 590 nm; λem 619 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 554 nm; λem 576 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

fluorescence

-

suitability

suitable for fluorescence

suitability

-

suitability

suitable for fluorescence, suitable for gel electrophoresis

suitability

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Application

Atto 590 is a new label with high molecular absorption (120.000) and quantum yield (0.80) as well as sufficient stoke′s shift (excitation maximum 594 nm, emission maximum 624 nm). Atto 590 has been coupled to streptavidin to enable all kinds of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays. Streptavidin conjugation of Atto 590 does not significantly change the labels spectral data regarding excitation and emission maxima. Given the optimized Dye/Protein-ratio molar absorbance and quantum yields remain high within application. Conjugates of our Atto dyes generally do not show strong fluorescence in unbound state, but fluorescence is boosted again as soon as they are bound to biotin. This may help overcome problems by unspecific binding which may sometimes appear with streptavidin based assays in special cases.

Analysis Note

free of unconjugated dye

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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