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412M-1

Sigma-Aldrich

Caveolin-1 (2297) Mouse Monoclonal Antibody

NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

culture supernatant

antibody product type

primary antibodies

clone

2297, monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (412M-14)
vial of 0.5 mL concentrate (412M-15)
bottle of 1.0 mL predilute (412M-17)
vial of 1.0 mL concentrate (412M-16)
bottle of 7.0 mL predilute (412M-18)

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100

isotype

IgG1

conjugate

unconjugated

control

Ewing’s sarcoma, mesothelioma

shipped in

wet ice

storage temp.

2-8°C

visualization

membranous

General description

Caveolin-1 (CAV-1) is a cell membrane-associated structural component of flask-shaped plasma membrane invaginations termed caveolae. CAV-1 is expressed at different levels in different tissues, with the highest in adipocytes, endothelial cells, fibroblasts, and mesothelial cells. Anti-CAV-1 is useful in assisting in the identification of epithelioid mesothelioma. A study by Amatya et al. indicates CAV-1 expression in all 80 (100%) epithelioid mesotheliomas, whereas only 6/80 (7.5%) of the lung adenocarcinomas were positive for CAV-1. Staining in most mesotheliomas was strong and diffuse when compared with the weak, focal staining (no more than 1% of the tumor cells) seen in the lung adenocarcinomas, anti-CAV-1 is considered a very useful marker for assisting in discriminating between these two malignancies. They also indicated that anti-CAV-1 is comparable to other mesothelioma markers such as anti-calretinin and anti-D2-40 (podoplanin) that are commonly used to assist in the differentiation between epithelioid mesotheliomas and lung adenocarcinomas. Anti-CAV-1 is also useful in identification of Ewing sarcoma/PNET. Literature indicates CAV-1 expression in 368 (96%) of 383 cases of Ewing sarcoma/PNET. In summary, anti-CAV-1 is very useful in the differentiation of epithelioid mesothelioma from lung adenocarcinoma and identification of Ewing sarcoma/PNET.

Quality


IVD

IVD

IVD

RUO

Linkage

Caveolin-1 Positive Control Slides, Product No. 412S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

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Other Notes

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Legal Information

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Certificate of Analysis

Certificate of Origin

Antonio Llombart-Bosch et al.
Virchows Archiv : an international journal of pathology, 455(5), 397-411 (2009-10-21)
Ewing's sarcoma (ES)/peripheral neuroectodermal tumor (PNET) are malignant neoplasms affecting children and young adults. We performed a study to typify the histological diversity and evaluate antibodies that may offer diagnostic/prognostic support. In total, 415 cases of genetically confirmed paraffin-embedded ES/PNET...
Alex W Cohen et al.
Physiological reviews, 84(4), 1341-1379 (2004-09-24)
Although they were discovered more than 50 years ago, caveolae have remained enigmatic plasmalemmal organelles. With their characteristic "flasklike" shape and virtually ubiquitous tissue distribution, these interesting structures have been implicated in a wide range of cellular functions. Similar to...
Vishwa Jeet Amatya et al.
Histopathology, 55(1), 10-19 (2009-07-21)
The incidence of mesothelioma is increasing in Europe, Japan and other developing countries. There is difficulty in the accurate diagnosis of mesothelioma and its differentiation from lung adenocarcinoma. Mesothelioma shows a complex immunohistochemical profile. Therefore, the use of a immunohistochemical...

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