41639

Sigma-Aldrich

Dimethyl sulfoxide

BioUltra, for molecular biology, ≥99.5% (GC)

Synonym(s):
DMSO
Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein/REAXYS Number:
506008
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

grade

for molecular biology

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

product line

BioUltra

assay

≥99.5% (GC)

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

impurities

Dnases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% free acid (as CH3COOH)

evapn. residue

≤0.005%

color

colorless

refractive index

n20/D 1.479 (lit.)
n20/D 1.479

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

cation traces

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.5 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤1 mg/kg
Ni: ≤0.5 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

SMILES string

CS(C)=O

λ

neat

UV absorption

λ: 280 nm Amax: 0.22

suitability

suitable for molecular biology

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Dimethyl sulfoxide (DMSO) is an effective aprotic solvent. It is colorless, odorless and hygroscopic in nature. DMSO enhance the permeability of various hydrophilic and lipophilic permeants. It is one of the widely used solvents in the laboratory.

Application

Dimethyl sulfoxide (DMSO) has been used as a vehicle for test compounds in various experimental studies. It has also been used as a solvent during amyloid β (Aβ) aggregation protocol for resolubilization of peptide to maintain its dissociated state.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Other Notes

Polybrene / DMSO-assisted gene transfer

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NA 1993 / PGIII

WGK Germany

WGK 1

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificate of Analysis

Certificate of Origin

  1. What is the molarity of DMSO?

    DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

  2. What is the DMSO dissolved in?

    DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

  3. Where does DMSO come from?

    DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

  4. Does DMSO have any synthetic utility?

    DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

  7. What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)?

    DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is Sure/Seal packaging?

    The Aldrich Sure/Seal packaging system is useful in the handling and storage of air-sensitive reagents.

R Aubin et al.
Methods in molecular biology (Clifton, N.J.), 7, 35-43 (1991-01-01)
Efforts to expand the current repertory of cell types amenable to transfection have often been thwarted by a common obstacle-namely, the low tolerance displayed by the recipient cells toward the gene-transfer regimen itself. As a result, several laboratories have turned...
Developmental toxicity assay using high content screening of zebrafish embryos
Lantz-McPeak S, et al.
Journal of Applied Toxicology, 35(3), 261-272 (2015)
Amyloid beta oligomers induce neuronal elasticity changes in age-dependent manner: a force spectroscopy study on living hippocampal neurons
Ungureanu A, et al.
Scientific reports, 6, 25841-25841 (2016)
The use of dimethylsulfoxide as a vehicle in cell culture experiments using ovarian carcinoma cell lines
Rodriguez-Burford C, et al.
Biotechniques, 78(1), 17-21 (2003)
Topical and Transdermal Drug Delivery
Handbook of non-invasive drug delivery systems: science and technology (2009)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.