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41784

Atto 647 maleimide

BioReagent, suitable for fluorescence, ≥90% (coupling to thiols)

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About This Item

UNSPSC Code:
12352111
NACRES:
NA.32

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product line

BioReagent

Quality Level

100

assay

≥90% (coupling to thiols)

form

solid

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 647 nm; λem 669 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

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1 of 4

This Item
806616849972998
Sigma-Aldrich

Sigma-Aldrich

41784

Atto 647 maleimide

Sigma-Aldrich

Sigma-Aldrich

80661

Atto 655-maleimide

Sigma-Aldrich

Sigma-Aldrich

68499

Atto 532 maleimide

Sigma-Aldrich

Sigma-Aldrich

72998

Atto 594 azide

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

fluorescence

λex 647 nm; λem 669 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 663 nm; λem 684 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 532 nm; λem 553 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 601 nm; λem 627 nm±10 nm in 0.1 M phosphate pH 7.0

form

solid

form

powder

form

powder

form

solid

assay

≥90% (coupling to thiols)

assay

≥90% (coupling rate)

assay

≥90% (coupling to thiols)

assay

≥80.0% (HPCE)

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

Atto 647 belongs to a new generation of fluorescent labels for the red spectral region with high molecular absorption (120.000) and quantum yield (0.20) as well as sufficient stoke′s shift (excitation maximum 645 nm, emission maximum 669 nm). Atto 647 is characterized by a high photostability. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Atto The dye is very stable under physiological pH-values and in buffers up to pH 8.5. However, it slowly degrades at higher pH. Atto 647 maleimide shows excellent solubility in polar solvents like DMF, DMSO or acetonitrile. After coupling to a substrate the dye moiety is electrically neutral.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN9639
BCCN4638
BCCN2278
BCCN0575
BCCM0682

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Daniel Puntener et al.
Journal of virology, 85(1), 481-496 (2010-11-05)
Human adenoviruses (Ads) replicate and assemble particles in the nucleus. They organize a linear double-strand DNA genome into a condensed core with about 180 nucleosomes, by the viral proteins VII (pVII), pX, and pV attaching the DNA to the capsid.
Duncan Hill et al.
Biomedical microdevices, 13(4), 759-767 (2011-05-12)
This paper presents an overview of development of a novel disposable plastic biochip for multiplexed clinical diagnostic applications. The disposable biochip is manufactured using a low-cost, rapid turn- around injection moulding process and consists of nine parabolic elements on a
Laura Pastorino et al.
Journal of colloid and interface science, 357(1), 56-62 (2011-02-26)
The protein collagen is the major component of connective tissue and it is involved in many biological functions. Its degradation is at the basis of different pathological processes. The up-regulated expression of matrix metalloproteinases and the down-regulated expression of their
Thorben Cordes et al.
Physical chemistry chemical physics : PCCP, 13(14), 6699-6709 (2011-02-12)
Modern fluorescence microscopy applications go along with increasing demands for the employed fluorescent dyes. In this work, we compared antifading formulae utilizing a recently developed reducing and oxidizing system (ROXS) with commercial antifading agents. To systematically test fluorophore performance in
Andreas Bruckbauer et al.
Biophysical journal, 93(9), 3120-3131 (2007-07-17)
We have developed a new method, using a nanopipette, for controlled voltage-driven delivery of individual fluorescently labeled probe molecules to the plasma membrane which we used for single-molecule fluorescence tracking (SMT). The advantages of the method are 1), application of
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