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41784

Sigma-Aldrich

Atto 647 maleimide

BioReagent, suitable for fluorescence, ≥90% (coupling to thiols)

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NACRES:
NA.32

product line

BioReagent

Quality Level

Assay

≥90% (coupling to thiols)

form

solid

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 647 nm; λem 669 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

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Atto 647 maleimide

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-
assay

≥90% (coupling to thiols)

assay

≥90% (coupling to thiols)

assay

≥90% (coupling to amine, HPLC)

assay

≥90% (HPCE)

form

solid

form

powder

form

powder

form

powder

fluorescence

λex 647 nm; λem 669 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 532 nm; λem 553 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 681 nm; λem 713 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 643 nm; λem 665 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

Atto 647 belongs to a new generation of fluorescent labels for the red spectral region with high molecular absorption (120.000) and quantum yield (0.20) as well as sufficient stoke′s shift (excitation maximum 645 nm, emission maximum 669 nm). Atto 647 is characterized by a high photostability. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Atto The dye is very stable under physiological pH-values and in buffers up to pH 8.5. However, it slowly degrades at higher pH. Atto 647 maleimide shows excellent solubility in polar solvents like DMF, DMSO or acetonitrile. After coupling to a substrate the dye moiety is electrically neutral.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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