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42247

Sigma-Aldrich

Atto 647N DOPE

suitable for fluorescence

Synonym(s):
1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 647N
NACRES:
NA.32

Assay

≥80.0% (HPCE)
≥90.0% (HPLC)

Quality Level

manufacturer/tradename

ATTO-TEC GmbH

λ

in chloroform/methanol

UV absorption

λ: 643.0-649.0 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

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manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

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manufacturer/tradename

ATTO-TEC GmbH

manufacturer/tradename

ATTO-TEC GmbH

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

λ

in chloroform/methanol

λ

in chloroform/methanol (8:2)

λ

in chloroform/methanol (8:2)

λ

-

UV absorption

λ: 643.0-649.0 nm Amax

UV absorption

λ: 627-633 nm Amax

UV absorption

λ: 643-649 nm Amax

UV absorption

-

General description

Atto 647N belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, excellent fluorescence quantum yield, high photostability, excellent ozone resistance, good solubility, and very little triplet formation. Atto 647N is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.
In common with most Atto-labels, absorption and fluorescence are independent of pH in the range of 2 to 11, used in typical applications. As supplied Atto 647N consists of a mixture of two isomers with practically identical absorption and fluorescence properties.

Atto-Dye Labeled Phospholipids

Sigma-Aldrich offers a variety of glycero-phospholipids carrying one or two fatty acid groups (lipophilic groups) and a phosphate ester residue (hydrophilic group). They are labeled at the hydrophilic head group. After incorporation of the phospholipid into a membrane the fluorophore is located at the water/lipid interface of the membrane. We currently provide 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), palmitoyl-sn-glycero-phosphoethanolamine (PPE), and 1,2-dimyristoyl-sn-glycero-3-phospho-ethanolamine (DMPE) labeled with Atto-dyes.

find more information here

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Analysis of replication factories in human cells by super-resolution light microscopy.
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