42424

Sigma-Aldrich

Atto 550

for fluorescence, ≥90% (HPCE)

MDL number:
NACRES:
NA.32

Quality Level

grade

for fluorescence

assay

≥90% (HPCE)

fluorescence

λex 554 nm; λem 574 nm in 0.1 M phosphate pH 7.0

storage temp.

−20°C

General description

Atto 550 is a new label with high molecular absorption (120,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. Atto 550 can be used with similar excitation source and fluorescence filters as Cy3® and is characterized by a high photostability.

find more information
here

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
CAB-O-SIL is a registered trademark of Cabot Corp.
Cy3 is a trademark of Cytiva

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
Antibody transfection into neurons as a tool to study disease pathogenesis.
Douglas JN, Gardner LA, et al.
Journal of Visualized Experiments, 26, 4154-4154 (2012)
Rula Masoud et al.
ACS nano, 6(7), 6272-6283 (2012-06-06)
We present a test case example of a detailed single-molecule fluorescence study of one of the most sophisticated and complex DNA devices introduced to date, a recently published autonomous bipedal DNA motor. We used the diffusion-based single-molecule Förster resonance energy...
Robert H Meltzer et al.
Lab on a chip, 11(5), 863-873 (2011-01-21)
Rapid, specific, and sensitive detection of airborne bacteria, viruses, and toxins is critical for biodefense, yet the diverse nature of the threats poses a challenge for integrated surveillance, as each class of pathogens typically requires different detection strategies. Here, we...
Rahul Roy et al.
Nature methods, 5(6), 507-516 (2008-05-31)
Single-molecule fluorescence resonance energy transfer (smFRET) is one of the most general and adaptable single-molecule techniques. Despite the explosive growth in the application of smFRET to answer biological questions in the last decade, the technique has been practiced mostly by...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.