43208

Sigma-Aldrich

Atto 590-Biotin

BioReagent, suitable for fluorescence, ≥90.0% (HPCE)

Synonym(s):
Biotin-Atto 590
Empirical Formula (Hill Notation):
C52H65ClN6O10S
Molecular Weight:
1001.62
MDL number:
NACRES:
NA.32

Quality Level

product line

BioReagent

assay

≥90.0% (HPCE)

fluorescence

λex 590 nm; λem 625 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto 590 is a new label with high molecular absorption (120.000) and quantum yield (0.80) as well as sufficient stoke′s shift (excitation maximum 594 nm, emission maximum 624 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Biotin conjugates can be used in applications like ELISA or immunohistochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Shira Halivni et al.
ACS nano, 6(3), 2758-2765 (2012-02-09)
Fluorescence resonance energy transfer (FRET) involving a semiconductor nanoparticle (NP) acting as a donor, attached to multiple acceptors, is becoming a common tool for sensing, biolabeling, and energy transfer applications. Such nanosystems, with dimensions that are in the range of...
Andrea D Lehmann et al.
Small (Weinheim an der Bergstrasse, Germany), 6(6), 753-762 (2010-03-06)
Iron-platinum nanoparticles embedded in a poly(methacrylic acid) (PMA) polymer shell and fluorescently labeled with the dye ATTO 590 (FePt-PMA-ATTO-2%) are investigated in terms of their intracellular localization in lung cells and potential to induce a proinflammatory response dependent on concentration...
Ronit Freeman et al.
Nano letters, 10(6), 2192-2196 (2010-05-21)
Semiconductor quantum dots (QDs) are used for the optical analysis of casein kinase (CK2) or the hydrolytic activity of alkaline phosphatase (ALP). Two schemes for the analysis of CK2 by a FRET-based mechanism are described. One approach involves the CK2-catalyzed...
D Wildanger et al.
Journal of microscopy, 236(1), 35-43 (2009-09-24)
The advent of supercontinuum laser sources has enabled the implementation of compact and tunable stimulated emission depletion fluorescence microscopes for imaging far below the diffraction barrier. Here we report on an enhanced version of this approach displaying an all-physics based...
Mariela M Marani et al.
Journal of combinatorial chemistry, 11(1), 146-150 (2008-12-17)
To screen one-bead-one-compound (OBOC) combinatorial libraries, tens of thousands to millions of compound beads are first mixed with a target molecule. The beads that interact with this molecule are then identified and isolated for compound structure determination. Here we describe...
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