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43410

Sigma-Aldrich

β-Nicotinamide adenine dinucleotide hydrate

≥95% (HPLC)

Synonym(s):
β-DPN, β-NAD, Coenzyme 1, Cozymase, DPN, Diphosphopyridine nucleotide, NAD, Nadide
Empirical Formula (Hill Notation):
C21H27N7O14P2 · xH2O
CAS Number:
Molecular Weight:
663.43 (anhydrous basis)
EC Number:
MDL number:
eCl@ss:
39200202
PubChem Substance ID:
NACRES:
NA.55

Quality Level

Assay

≥95% (HPLC)

form

powder or crystals

impurities

≤4% water

color

white to faint yellow

solubility

H2O: 0.1 g/mL at 20 °C, clear
H2O: freely soluble

cation traces

Ca: ≤200 mg/kg
Cd: ≤50 mg/kg
Co: ≤50 mg/kg
Cr: ≤50 mg/kg
Cu: ≤50 mg/kg
Fe: ≤50 mg/kg
K: ≤50 mg/kg
Mg: ≤100 mg/kg
Mn: ≤50 mg/kg
Na: ≤1000 mg/kg
Ni: ≤50 mg/kg
Pb: ≤50 mg/kg
Zn: ≤50 mg/kg

absorbance ratio

A280/260 nm 0.22 ±0.02
A250/260 nm 0.83 ±0.03

storage temp.

−20°C

SMILES string

NC1=NC=NC2=C1N=CN2.O[C@@H]3[C@@H](COP(O)(OP(OC[C@@H](O4)[C@@H](O)[C@@H](O)[C@H]4[N+]5=CC=CC(C(N)=O)=C5)([O-])=O)=O)OC[C@@H]3O

InChI

1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1

InChI key

BAWFJGJZGIEFAR-NNYOXOHSSA-N

Gene Information

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This Item
N7381N1636N7004
assay

≥95% (HPLC)

assay

-

assay

≥99%

assay

≥96.5% (HPLC), ≥96.5% (spectrophotometric assay)

form

powder or crystals

form

powder

form

crystalline powder

form

powder

color

white to faint yellow

color

white to off-white

color

white

color

white to off-white

solubility

H2O: 0.1 g/mL at 20 °C, clear, H2O: freely soluble

solubility

-

solubility

-

solubility

-

cation traces

Ca: ≤200 mg/kg, Cd: ≤50 mg/kg, Co: ≤50 mg/kg, Cr: ≤50 mg/kg, Cu: ≤50 mg/kg, Fe: ≤50 mg/kg, K: ≤50 mg/kg, Mg: ≤100 mg/kg, Mn: ≤50 mg/kg, Na: ≤1000 mg/kg, Ni: ≤50 mg/kg, Pb: ≤50 mg/kg, Zn: ≤50 mg/kg

cation traces

-

cation traces

-

cation traces

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

β-Nicotinamide adenine dinucleotide hydrate has been used as a component of fibroblast cell culture media. It has also been used to study the viability of the cartilage constructs.
β-Nicotinamide adenine dinucleotide hydrate was used as a test compound for studying the structure and electrochemical properties of NADH electrocatalysts.

Biochem/physiol Actions

β-Nicotinamide adenine dinucleotide is a cofactor for alcohol dehydrogenase that serves as inhibitory neurotransmitter in visceral smooth muscle.
β-nicotinamide adenine dinucleotide (β-NAD) is a coenzyme present in most living cells. It is implicated in redox reactions and posttranslational modifications. β-NAD also acts as a substrate for various enzymes in several cellular processes.
Electron acceptor

Packaging

Packaged by solid weight.

Other Notes

This is the common form of NAD.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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